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ReviewQuestionsLec24 - mutant identified initially(in other...

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Review Questions for Lecture 24 1. What plant tissues did the researchers use to purify the first brassinosteroid? Why did the researchers name these steroids brassinosteroids? 2. List major physiological functions of brassinosteroids. 3. Explain why brassinosteroids had not been accepted by the majority of plant biologists as a new class of plant hormones for over 25 years despite many studies showed that brassinosteroids can affect many plant physiological processes? 4. What are the structural requirements for brassinosteroid to have biological activity? 5. Describe the mutant phenotype of the Arabidopsis det2 mutant. How was such a
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Unformatted text preview: mutant identified initially (in other words, was this mutant discovered in a targeted genetic screen for mutants that are defective in producing brassinosteroids)? 6. What was the initial discovery that led to the hypothesis that det2 mutant might be a steroid biosynthetic mutant? Explain an experiment that proved this hypothesis. 7. How were the bri1 ( brassinosteroid-insensitive 1 ) mutants discovered? What kind of protein does the Arabidopsis BRI1 gene encodes? 8. What is the main inhibitor protein in the brassinosteroid-signaling pathway? How does this negative regulator affect the plant steroid signaling?...
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