DNA Replicatio1 - 3. Dispersive replication involved the...

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DNA Replication DNA was proven as the hereditary material and Watson et al. had deciphered its structure. What remained was to determine how DNA copied its information and how that was expressed in the phenotype. Matthew Meselson and Franklin W. Stahl designed an experiment to determine the method of DNA replication. Three models of replication were considered likely. 1. Conservative replication would somehow produce an entirely new DNA strand during replication. 2. Semiconservative replication would produce two DNA molecules, each of which was composed of one-half of the parental DNA along with an entirely new complementary strand. In other words the new DNA would consist of one new and one old strand of DNA. The existing strands would serve as complementary templates for the new strand.
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Unformatted text preview: 3. Dispersive replication involved the breaking of the parental strands during replication, and somehow, a reassembly of molecules that were a mix of old and new fragments on each strand of DNA. The Meselson-Stahl experiment involved the growth of E. coli bacteria on a growth medium containing heavy nitrogen (Nitrogen-15 as opposed to the more common, but lighter molecular weight isotope, Nitrogen-14). The first generation of bacteria was grown on a medium where the sole source of N was Nitrogen-15. The bacteria were then transferred to a medium with light (Nitrogen-14) medium. Watson and Crick had predicted that DNA replication was semi-conservative. If it was, then the DNA produced by bacteria grown on light medium would be intermediate between heavy and light. It was....
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This note was uploaded on 11/29/2011 for the course BIO BSC1010 taught by Professor Gwenhauner during the Fall '10 term at Broward College.

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DNA Replicatio1 - 3. Dispersive replication involved the...

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