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Unformatted text preview: for conjugation. (Box 15.1 U tube experiment) E. Getting DNA by Transduction : F. Using bacterial mutagenesis 1. Common type: gene replacement in bacteria (Fig. 15.11 and 15.12) Page 2 Mutations Part II Bio119 a) Method for adding or deleting a gene b) Why do this? G. Random transposon mutagenesis (Fig. 15.17 and 15.18) 1. What is a transposon? a) An genetic element that moves from one site into another b) The Tn is has two inverted repeats c) Usually has some antibiotic resistance marker in between IR d) e) The Tn is also engineered without the transposase so that it wont transpose again. f) 2. Screen for mutants (takes longer to do) 3. Selection for mutants (harder to do with for Tn mutagenesis) Page 3...
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