15_outline_Mutate2 - for conjugation. (Box 15.1 U tube...

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Mutations Part II Bio119 I. Mutations by adding new DNA to the genome A. DNA can be taken up by prokaryotes and incorporated into their genomes B. DNA that comes in either can replicate on its own, or is recombined into the chromosome 1. To replicate on its own: 2. Otherwise, to persist it has to go into: 3. How this is done ( Figure 15.6 and 15.7): C. Getting DNA into cell by transformation: 1. Taking up plain old DNA 2. Natural Competence: story of Streptococcus pneumoniae (Fig 15.14) Page 1
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Mutations Part II Bio119 3. Artificially induced competence D. Getting DNA into a cell by Conjugation 1. Transfer of genes from one prokaryote to another by a mechanism involving cell-to-cell contact 2. What is transferred? 3. How conjugation works (Figure 15.9): 4. How scientists figured out cell-to-cell contact was required
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Unformatted text preview: for conjugation. (Box 15.1 U tube experiment) E. Getting DNA by Transduction : F. Using bacterial mutagenesis 1. Common type: gene replacement in bacteria (Fig. 15.11 and 15.12) Page 2 Mutations Part II Bio119 a) Method for adding or deleting a gene b) Why do this? G. Random transposon mutagenesis (Fig. 15.17 and 15.18) 1. What is a transposon? a) An genetic element that moves from one site into another b) The Tn is has two inverted repeats c) Usually has some antibiotic resistance marker in between IR d) e) The Tn is also engineered without the transposase so that it wont transpose again. f) 2. Screen for mutants (takes longer to do) 3. Selection for mutants (harder to do with for Tn mutagenesis) Page 3...
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This note was uploaded on 12/03/2011 for the course BIO 118 taught by Professor Staff during the Fall '08 term at Rutgers.

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15_outline_Mutate2 - for conjugation. (Box 15.1 U tube...

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