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exam2_key - 1 Define the following terms briefly You mayr...

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Unformatted text preview: 1. Define the following terms briefly. You mayr give an example if 1you think this would be helpful. but examples are no! required. {3D points} a. heteroohromatin HDML fim‘oin; Wmtin Mops-Ar hrijhly repairing: 011395 WM {5hr fiéfflf haw (Zerfirew’résr. {Chew} PFC» b. anti-oodon 3 “Meade {$0616 on {QUA- ‘Hw‘r WW5 of: Ler at (sign cm NEW on order is die fight tee/em fie”! we (were a. F: e 52:53:“ ‘ 4 s AFplasmel He he; (fiomblf'fil with 59W: genres 60m elk; Laden-1i Chreflisfiomafl Lg) d. oie Fad-w 3% age} 'jerflfi. on rile Smirk; etwml at DNA; 61:: pmsz-ifir .7- S e. nuoleosome A up} M- Map 0‘“ 0N7? “fir-Well orourwl Astana? W mflfdfl' +9 59% Dflflaf, (rm mwfi f. f—met A" Mk'é’fllkfl wihk "L EFF/V! 7912, ammo quJ WM 1“ .wprygl‘i ah; 'i'rWl-‘H‘mh F I es E. Giyen the following prokaryotic coding strand sequence. please answer the following questions: 35 pts. total a. What is the seguenoe and polarity of the template strand? {5 pts.} in. Where is the approximate transcriptional start site? {5 pts.) c. What is the sequence and polarity of the mRNA? {5 pts.) a. Translate the mRNA. indicating amino and carboxy termini of protein. {5 pie} a. Is the proteinipiar or nongolar overall? {5 pts.] t. Wl-iat is the complement to the mRNA {with polarity indicated}? (5 pts.) Q. Can you translate the complementary mRNA {does it have a start codoni. and if so. what would he the protein sequence? {5 pts. 3:“ 3 [I S'I‘TQTGJGCQQQQEEGGATGCTAAGCAWGAF orccegmreacccwrcca TECCGGCCGE' T _ 43m; i it till” __ snot “a I DU aimpims Ettortd (1T soTTTC F‘ 5&1“: 35‘ title Fleecech eiTecce @191 cc. Thqu aTT cc-{r_op.-r'h"r‘i'ee wag—r eeeTFi hem-e ("fl—HG": Cos—it‘s c. ‘5‘ I m Jimgfl-“Wiflml Sign 9M ii» bag-2.. ‘3de +1 0‘06?” r {3 miles W _ a 5' a await came-Lil" Ptflfiggfljgfigfil :3" H" d) WT yME-r mo: Hi‘S'PRED- sceahoe—Phflc Cacti a r? my“: to to (“it ii“! (P) the E) DAM UT. 4M F'I. HILL “ED-i cthem [11E {JOl'Kfl-I Tngflfi TU“ scam "is moi loos ' was! omit _ 9’1"!th a:th Li W“ PD‘M batman '3: Ni was? idiom oatml - [is Q) Corfipiewflmi at rnflbfii’i ‘ {slash "1 ‘1‘ W 5' Wflmfifl 371—th tree at; eras E“ cm hue} g3 \IEJ “Pu Cflfl Affinfi‘tqifl dim. COMPEMEN’ oi with} Dflflmlit 3mm; mmrm PINE-MI . __-:c '3’ i-tutETaitfi’a avg—5‘ E11- Lam-TRRTM: (mini —i .. ‘- iir-a " _ _ _ 3. Please annotate the replication bubble below. Show bidirectional replication (Le. at both replication forks}, including RNA primers, leading strandls}, lagging strandlst Dkazaki fragments, and polarity of all DNA strands (20 pls.) i / _ _ 4. Answer the following questions about the Meselson-Stahl experiment. You may use words or annotated drawings. whichever you prefer. a. What were the three hypotheses of DNA replication that were being tested by this experiment (you can just list them by name. or if you can't remember the exact name you can describe them}? [3 pts] b. Originally. bacteria were reared in '5“; in all subsequent generations. they were reared in 1"‘N. How many bands were expected to be seenr and of what weightrs}. in the first generation after changing the medium, for each of the three hypotheses? {9 1315) a. One generation in “N was not enough to tell between two of the three hypotheses. Accordinglyr bacteria were reared for an additional generation in MN. How many bands. and of what weightts). were expected for each of the three hypotheses in this generation? [El ptst “3 Conservative, Semrrsnseryahya, cliS'PtiFSlt’d 4, t J1 4”; :chi strands warmer 1.31:1 shims or} reality: m"- nrw shohds mgcmrr' 1 MW shay” aid and nfro '95 it romsrryafive“. L W bands 2‘ a. were to be scan :1” on: tighrrrtryryjnncl I N finer Lists! iFeritonsrryoh aw i humer USN) Ema-l “:3 band as . 0mg Ont, band ‘Eso tonsrryafiye 7715” IS Ir'iblf. Mean m t, 4mm . maflgggg H; dispgrfiwe MN and EN band; l‘tNm‘lJI‘bN _ band hfi'seen which (I cl I‘iw flhcjflfiilgflfifdbfmtfin Th; UH, bum WNW: m C) 2nd 3 [/ \ 65m was Ll-Sf‘ct tn cl ?{ J 11%an Holt: between armiron hire a r _ on rllJPFFEiVE. ['Dr'tSEt’VcrHvfl was fliraddy PYt‘ fi€MFflHon til armIC‘D 5. Draw the elongation step of translation in prokaryotes. You can assume that initiation has already started, so start with a polypeptide chain that is taro amino acids tong {MET and TRP}. add two amino acids, ARC-i [codon CGA} and PHE (oodon UUU], to the polypeptide chain. Thus your drawing shoutd hays three parts:@the starting state as defined shore with the polypeptide chain containing only MET TFtF:@ after W P, __ IJGG adding the first amino acid (ARE, oodon CGA}; [email protected] after adding the second amino ' acid tPHE, oodon uuui. rid : Mi . . “M .N’. 9" “f. hearse Be sure to Inc1ude theswe‘jm gs: tRNAs. an'nno acids, anticodons [giylude the .r' J, h 1 M k appropriate bases}, code): se the oodons specified eboye}, rnR Al’rnFtNA polarity, '9" 4' J J peptide bonds, ribosome wit A and P sites, and the direction of travel of th to some g a with respec - the mRNA {20 p13} 59-? W: e .:at are three madificatinns to eukaryatic mRilAs? What is the function at each? (15 Jr ./ f) Add gunman: 5' m1: 4» mfl-hflfl'l': : medEE/FI mrm. (kc/Ring Phflcc‘ I ” ll - T} 333% 4' add '5 and F0194 “1141'- Eth: MEN-ID: 3W Sifiiunfl; ffimnva excesaivathbna fibm Ir,th flung (Sena mdmfl TEE—1m”: *fiH-Egm’hvfi g'lwhinfifl' unfilmfl‘flh‘i' film [31? r541}: cLL-’ffl[email protected]fi F. A series of tour Hfr strains were studied for mapping purposes. Each gene is present exactlyr once on the bacterial chromosome. Based on the data below draw a map of the bacterial chromosome. with the genes in order. Alfiflr write out the complete bacterial genotype with all the genes in order for each Hfr strain. including the tocation of the F factor for each strain. You do not need to indicate whether alleles are + or -_ {ED points} B. In ——i— order tc detect gene transfer in bacteria. two different alieles cf the genes need tc be present in the cone: and the recipient. Imagine the transfer at the genes th recnine and maitase. Which alleles wculd the dcncr (HFR) and the recipient {F-J have far these genes tie. thr— vs. thr+; mai- us. mai+} and why? {10 pts] Jrh H F‘lg p F {'6} male) ‘41,?) mate m“?! 1h? GHE- ‘ff 1&1! a "E E- El Fiene‘Fy-x’efll' dr-_merm1fe {h +11 .6? 3m .. HFR “are Ci} tn “sz F“- +14“ (stemmed G3 He - J PJ'EM0‘J'YF' if?!" Ci-mgrzflct WM J59 aaflfiyfl GEHLE flrd- Edi-ed '5‘“ F- r1154 aha Ell- 9. Given this diagram and Bitl Alien! guest lemre. explain which quadrant has Ihe highest level at pubic acceptance. and which has the least. Give an em mple team he hypothetical] for ham. 1D p15. fly-J5 Qutirfinl FraMt-fi HM. L.qu at itlugirs w either-flit“:- WHEN} wtnk SHM'IrL felt-3’ -pqfifid’t glut Wtfmlfit'lrh'h wl the} lu: Ffifisffll arr-x fairway}. Limb; L a». qua-d: Mmttul’Jfih at, Hui .ww LIMIT; +0 calm-1. Farkinbahqfi 5r 'Mftn'ms F l. '1' Enhaflfleofic‘ter'flrhh E p W m5 quadrant (Outs! firm-4H a” bffilbfilmf It “iEhfif‘dC-f'pifi‘ “(Ehkmfin‘dn tt'trttvtAL:_-=t.‘{§ Stat-UL?! 31m. Agr- iiut-nttaf 01""r'fm "wtau 14 waist manhunt 11.5 H LI fats-3m! uL-Crwvx EM glhattrmh‘en 5 " lhn' E I m. 9.1”“? MAHI‘FLIIIHJH at HM Y-flwmsuw. Hui [Jami-E5 MET‘HSM mujl‘lt: arrwlL _ 1|}. Ccmpare and ccntrast rho-dependent us. rhc-independent [er intrinsic} transcripticn termination. Describe each mechanism in detail. Use a drawing if that wculd be helpful. but a drawing is net required (19 pts.) (D " Jeffldfink liken H‘ acts like iti'tcesti. ...
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