lacproblemsinclass

lacproblemsinclass - in the operon and their phenotypes are...

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1. For each of the following partial diploid genotypes of E. coli , state whether 1) β -galactosidase is made and, if β -galactosidase is made, 2) and if so whether expression is inducible or constitutive. In this problem, the genes on the plasmid are on the left. If a gene is not listed, you may assume that it is wild type. a. lacZ + lacY - / lacZ - lacY + b. lacO c lacZ - lacY + / lacZ + lacY - c. lacP - lacZ + / lacO c lacZ - d. lacI - lacP - lacZ + / lacI - lacZ + e. lacI + lacP - lacY + / lacI - lacY - 2. In a theoretical operon, A, B, C, and D represent the repressor, promoter, operator, and a structural gene, but not necessarily in that order. This operon breaks down a theoretical molecule (tm). A series of mutants
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Unformatted text preview: in the operon and their phenotypes are described in the table below. For partial diploids, F' denotes the plasmid. AE = active enzyme, IE = inactive enzyme, NE = no enzyme. Genotype tm present tm absent A+B+C+D+ AE NE A-B+C+D+ AE AE A+B-C+D+ NE NE A+B+C-D+ IE NE A+B+C+D- AE AE A-B+C+D+ / F'A+B+C+D+ AE AE A+B-C+D+ / F'A+B+C+D+ AE NE A+B+C-D+ / F'A+B+C+D+ AE + IE NE A+B+C+D- / F'A+B+C+D+ AE NE a. Is the operon inducible or repressible? Why, in one sentence? b. Make a table assigning A, B, C, and D to the four parts of the operon listed in the question. Explain your reasoning in a separate paragraph. Be SUCCINCT....
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