PS10 - -3 to 10-5 Another 7 do not revert spontaneously at...

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Problem Set 10 Due: Thursday 17 November 10 POINTS Required problems: 1. In what two different ways do bacteria "sense" the amount of trp in the environment? List both, and describe *one* mechanism in detail. 2. What are two hypotheses for the functions of RNA silencing (i.e. RNAi)? Be brief. 3. A major area of research in the space program is the estimation of mutation rates in space ( e.g. , on the space station). a. What type of mutagen do you think NASA is worried about? What type of mutations would it cause? b. What kind of experiment would you use to estimate mutation in space, assuming you could create any artificial conditions you wanted (gravity, heat, etc)? Write the name of the experiment. Briefly explain the appropriate control you would use for your experiment, assuming you could create any artificial environment you wanted. 4. Twenty spontaneous white-eye mutants of Drosophila are examined individually to determine their reversion frequencies. Of these, 11 revert spontaneously at a frequency of 10
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Unformatted text preview: -3 to 10-5 . Another 7 do not revert spontaneously at detectable frequency, but can be induced to revert at frequencies ranging from 10-5 to 10-6 by treatment with alkylating agents. The remaining 2 cannot be made to revert under any conditions. Answer the following questions, and explain your answers . a. Which class of mutants would you suspect result from transposable element insertion? b. Which class of mutants are probably missense mutations? Name one mutagen or process that causes missense mutations. c. Which class of mutants are probably deletions? Name one mutagen or process that causes deletions. 5. Given what you know about UV-induced mutations and their repair, would it be a good idea to be in the sun for short periods prior to a beach trip? Why or why not? Would your answer be the same or different if you were a bacterium? Optional problems: 16: 2, 4, 8, 14, 22, 26, 28, 30....
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This note was uploaded on 12/02/2011 for the course PCB 3063 taught by Professor Marta during the Fall '08 term at University of Florida.

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