Biolog 16 - Chapter 16: Genomes I. Restriction enzymes and...

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Chapter 16: Genomes I. Restriction enzymes and recombinant DNA A. Cut double-stranded DNA at a specific base sequence between 4 and 8 base pairs B. Molecule that consists of base sequences from 2 or more organisms of the same species (DNA ligase) II. Reverse transcriptase A. Viral enzyme that uses single strand of mRNA as the template to make cDNA for cloning B. Catalyzes bonding of free nucleotides to strand of complementary DNA or cDNA on mRNA III. Genome A. All the DNA in a haploid number of chromosomes that characterize the species B. A collection of gene library is collected and researchers use probes to determine the gene they want to clone C. Nucleic acid hybridization occurs when there is base pairing between more than one DNA or RNA D. A primer is the short stretch of synthetic DNA made in cloning E. Polymerase chain reaction is to replicate a gene DNA fragment IV. Automated DNA sequence A. T*, C*, G*, A* are the modified nucleotides that would halt strand assembly. B. DNA sequencing rapidly reveals order of nucleotide in DNA fragments
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Biolog 16 - Chapter 16: Genomes I. Restriction enzymes and...

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