Exam2Answers - 2 1. In the dihybrid cross shown below,...

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Unformatted text preview: 2 1. In the dihybrid cross shown below, genes A and VB behave according to Mendel's two laws. In addition, allele A is completely dominant to a, allele ijyis completely dominant to and the A and genes affect different traits. '4"; P AAgg x aaGG F1 AaGg (self) F2 A. What are the gamete genotypes produced by the F1? Circle your answer (2 pts.) A6 A3: a: 4;} «.2 B. At what Frequency is each 9' amete type in question A above produced? Circle your answer (3 pts.) - "7’ (q . AC) 8 A; :1; T1; \ Q (Tar-y {/‘l I J / _’ f .‘ f ' j" C. How would your answer to question B above change if the alleles do not obey Mendel’s 2“‘1 law because they are linked with a recombination Frequency of 5%? Circle your answer (10 pts.) 0' H, ,. y l «f A , W “V W "‘7 -'x AC . 9 r6}. will 19 if: '7 = (-7.! ,.,, 0 l i! (Q: /{‘_) Fl ._ 70 3'0 l ‘v , 2. Consider the Following testcross (A andB control different traits. fl is completely dominant to "a? and B] is completely dominant to l3): } EF / eF X eF / eF i EF / ef 99 eF / et 99 EF / ef 1 at / 2F 1 A. What is the recombination Frequency (RF) between the "E" and "F" genes? Circle your answer. (5 pts.) i 2 Of.) B. During a typical meiosis (in other words, in most meiosis events), how many cross-overs occur between the "E" and "F" genes? (Your answer should be an integer — circle it.) (5 pts.) ZERC) C. Are the "E" and "F" genes on different chromosomes? Yes (circle one) (5 pts.) D. IF instead of doing a testcross you self—crossed EF/eF, what would be the Frequency of the "eeFF" genotypic class in the F2? Circle your answer (5 pts.) n crsgvs ll- T’ aggnjl“ A \“ 4 3. Four homozygous mutant lines of Fruit Flies (labeled 1—4) each have the same mutant phenotype: four wings instead of the normal two. Each line is homozygous For an amorphic mutation in a single autosomal gene. The lines were intercrossed, and the phenotypes of the resulting F1 Flies are shown in the table below. “+” indicates that the F1 had two wings, and “—” indicates that the F1 had Four wings. 1 2 t 4 no (£21331: si TEL? é‘i‘.‘ r: k with” .W , 1 — + + .. i i» 2. + ~— s. + g i l v" {4 x 3 t .— ~ + «if 4 + + + _ W A. What is the w describes this kind of test? W (5 pts) (lw'éljrif-M; i B. How many different genes are mutant among the 4 lines? “W? (5 pts) (your answer should be an integer - circle your answer) k) C. Which lines contain mutations in the some genes? CW. (5 pts) (x) J D. From the description of the alleles and the mutant phenotype above, what would you infer the normal Function oF these genes to be? Circle your answer. (5 pts) . r - {ii-‘33:“! f. 1 .xr“ 4 \ E. What would you expect the mutant phenotype of m-/m+ heterozygotes to be if m- were a dominant hygermorphic allele of one of the above genes? Circle your answer. (5 pts) M ii" f” a; ; || .7, 3 5 4. You are growing a strain of auxotrophic leu— (cannot synthesize the amino acid Leucine) E. coli bacteria in a Flask of minimal media supplemented with Leucine. A. To identify leu+ prototrophs that may have arisen by spontaneous mutation, you would need Petri plates with what kind of media? (2 pts.) Wm Circle one: minimal media-7“ minimal+Leucine complete media B. Is the method you are usingmi‘n A a screen or a selection? (3 pts.) Circle one: screen (selection\ 5. A. Taking into account the Wobble Rules and the Genetic Code Table, what is the minimal number of distinct tRNA genes required to recognize all Praline codons? (Your answer needs to be a single number - circle your answer.) (5 pts.) B. What are the anticodon sequences of the tRNAs that the genes in the answer to part A above generate? (You must indicate the 5' ends to get credit For your answe‘r- CIRCLE your answer.) (5 pts.) v. I“ '- n a 3:) _l (iffy a j . g} “"T (:7 (3'7 g , ! _ x If r I if T .i ~\, f e_ 5! J c; ",1 6. A tRNA with the anticodon sequence 5'UAU cannot exist. Why not? (10 pts.) circle your answer Z { iwa if. ,4 - ‘f E v” ” I . l ‘ ‘ r. . ‘- I ‘. - ;' :gf/zuft- {we} H- ._ 7",}, {I J _ 1‘ _ I 9 \ r ‘ " i" a - is l‘ .‘i '= 1% i’<" i "- ' I I {r If”. If.“ I - K sr mm»- k a ‘ h; ..__l. _.« ~..,. ,.. , 'I "a! 7. IF you cross normal Female FruhL Flies with normal males, each Female will produce abou’r 200 F1 progeny. Le’r's say you se’r up a cross of 5 Females with 5 males and obfained 1000 F1 Flies. A. Among ’rhe Fl, how many do you expecl’ to be males and how many Females? (1 pt.) circle your answer \ A") “500 " f . I’ ~-‘ \V i_ A ’ * a.- Tan ,4. B. How would your answer ’ro "A" change iF you Fed The Females ’rhe mu’ragen EMS beFore maling ’rhem wi’rh the males? (9 pfs.) circle your answer £13548 '1 . . . VUK’. {is} ‘K r x ‘v LA” ‘ i a i ,I r . . A r: i}; . “EM! 5U y "“ " , 9"" .. ~ _4v..- - e \s l I " i w p HS 0 ' ,‘J‘. {24 s /’/_, /l“ 5/ .5 ;‘ '5. " 1‘ I, I , 11“ .9 ("it ( r} 9/ I“? ,p‘ I]! i y l“ i 8. Consider ’rhe pedigree diagram below For an aufosomal dominan’r disease fha’r is 100% penefran’r. In order +0 show symptoms oF +his disease, every soma’ric cell in your body musf have fhe mu’ran’r allele. T lwl k, i” “ , .. ,.‘ ’ i ' Arow p52: y it“. mm + 'r“ T n ,4 ,3 l "u l ._ l H , r ?" i -T—Y-v-v' ; . L I I [m {is If W 1. Where did fhe disease allele fha’r appears in general’ion III mos‘r likely come From? (be as Specific as you can be) (5 pfs.) cnrcle your answer DUllua uuua I IV! I 077/175 odud we HEiI’WV MI nor Mir. La,“ a {w 3 a you Could 468 am We #17:: , if-mu/d 5 a? 1%ng {(4 Wombmaho-n a)ch 0i #1114" a” F 6/13,} $0 “#11121” (Of) 057 [ha/Mes 4416, 4974’ QUE/a 01“FF-"€”(e' . If fl‘li'Qmaf‘chad 394ch mm‘ repmr‘éhj {L5 before Mefoms a 5m 55/151]:sz 7%; am. ‘2‘ :3: (mid, mutt (gag MM) \ , Wha’r is unuSual about +he Neurospora oc’rad above? ...
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Exam2Answers - 2 1. In the dihybrid cross shown below,...

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