Exam3Answers - 2 1 The sequence of a cloned Fragmen’r of...

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Unformatted text preview: 2 1. The sequence of a cloned Fragmen’r of DNA was defermined using lhe dideoxy me’rhod. A par’r of fhe au’roradiogram of l'he sequencing gel is shown here. The larger molecules are al’ The top (BIG) and smaller ones are al the bo’rlom (SMALL). dd‘A ddG ddc ddT - _ ""77. I'll-(,1 __ l ' "3:. .— f7 — EM]! 3" 5/ _ are-[7,. ;' _ jg.’ _ — _ fl 7‘ fl — 1‘;‘—l///~ " ’ — r T" :MNL Deduce ’rhe nucleofide sequence of the single-sfranded DNA nucleo’ride chain fha’r was The template For DNA syn’rhesis. Label l'he 5' and 3' ends, and circle your answer (25 pfs.) V—a ~Ar— er' / f ~ ‘,.4—-,-—/‘-*"' « ' ‘1' ii/‘./I:‘/('."| *- fif'fig’ - " -‘ wen? /;’~. ” /' A?“ .2 . f,’ * ~ ~ ’1 (JRy 3 2. Cystic Fibmus is caused by autosomal recessive loss-oF—Function mutations in the CF gene. One common disease allele is a point mutation that results in an RFLP, as shown below (R=Eco R1): Samples oF genomic DNA obtained From a Fetus (F) and her parents (M and P) were digested to completion with Eco RI and then subjected to gel electrophoresis Followed by Scuthern blotting and hybridization with a radioactively labeled probe designated “CF probe" in the diagram above. The autoradiographic results are shown in the Figure below. (Larger moleCules are at the top.) F M P l- - —I A. Do either oF the parents have the disease? :’\@NO (circle one) (5 pts.) - B. Which parent is heterozygous For the disease allele? i"/_\/ M BOTH (circle one) (5 pts.) C. Will the Fetus have the disease? YES § ND (circle one) (10 pts.) *~ 4 3. Consider the pedigree diagram below. The affected individuals in the diagram have a disease carried by a dominant allele of an autosomal gene, D. The disease phenotype is 100% penetrant. The disease gene is linked to the gene I that controls ABO blood type. A. Circle the symbols For the people in the diagram who are most likely recombinants. (15 pts.) 8. Using all of the data in this diagram, calculate the recombination Frequency you observe between the “I” and “D” genes. Circle your answer. (15 pts.) r/ 2 J 5 4. The particular SSR locus shown below (x=number of repeats) is linked to a dominant autosomal disease gene allele, D. A pedigree diagram For a family with the disease is also shown below. PCR primer 1 —4 (Ac) x — PCR primer 2 PCR products using primer1 and primer 2 A. Indicate the most likely configuration oF the normal (d) and mutant (D) alleles of the disease gene with respect to the particular SSR alleles in each of the two parents. In other words - diagram the relevant pair of homologous chromosomes for each parent. 2| / PC: ' ‘2' W circleWUO pts.) F0 0. 55%? ‘3'. ,3 p: B. Circle anyone in the diagram who appears to be a recombinant. (10 pts.) C. Using only the data in this diagram, what is the recombination Frequency between the SSR locus and gene D? (5 pts.) ' [/3 BONUS QUESTION On July 20 of this year (2011), Google tried to honor Gregor Mendel on his birthday by posting the following "Google Doodle" on their webpage: List all the ways you can think of in which this Figure is wrong or misleading in terms of either standard genetic nomenclature, the expectations For a sell-'- Fertilization of a hybrid, or the actual ways in which genes might determine the specific phenotypes seen. - I. Genotypes are fr’?f‘:0p"-9id #11063 showid log d.pio,d _ 02. Caawl‘a was not wjgee flirt/135mm @1ij A 3. Green color 01’ Peaks IS dug Lo hon-9:93.331: ‘6.” a titressrué OMdfib/i [-1 i3 [ape—94.569 I 1 LI r I 4. IS {AY‘CIEQV UJ‘MLWa be; Udrfrffr] IS UPPErCa-gt, orJDnger—Z' bl W WOLFE 8%ch"; 5) 3"} V4470 of ya/awifi‘i/Mfl/ZEM: 1407,— it?) as in. *1/10 d: a f (a. gal Cofor is deiflfmflwrd we Qibfiz it‘d-Md” {7'3" ' 3+ is um Jae t 7" 9/ (55‘5" 93ft?!” arr/m . I I r I 4:0“ it". in a {W "" '2 p6 of 74:12 0f ilk/1 ' r ‘n‘m ; «r ‘ g Pod Coir)! cliff? JAE/n “3’01 _ i g t x ‘ diggrgwf' fled/Lg, Ii . M \l ...
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Exam3Answers - 2 1 The sequence of a cloned Fragmen’r of...

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