Absorption Spectrum protocol

Absorption Spectrum protocol - absorb light best at...

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Sara O’Donnell Absorption Spectrum Purpose: In order to determine the wavelengths of light useful for photosynthesis, we will determine the absorption spectrum of five pigments from 400nm to 700nm, taking readings at 20nm intervals in a spectrophotometer. Scientific Hypothesis: Pigments will absorb light at the wavelength that best corresponds to the color they project. Null Hypothesis: Pigments will not absorb light at the wavelength that best corresponds to the color they project. Predictions: 1. If Chlorophyll b is placed in a cuvet and put in an spectrophotometer, than it will absorb light best at wavelengths 525nm-560nm. 2. If Chlorophyll a is placed in a cuvet and put in a spectrophotometer, than it will absorb light best at wavelengths 450nm-500nm. 3. If Xanthophyll a is placed in a cuvet and put in a spectrophotometer, than it will absorb light best at wavlengths 560nm-580nm. 4. If Xanthophyll b is placed in a cuvet and put in a spectrophotometer, than it will
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Unformatted text preview: absorb light best at wavlengths 560nm-580nm. 5. If Carotene is placed in a cuvet and put in a spectrophotometer, than it will absorb light best at wavlengths 600-700nm. Rationales: 1. Chlorophyll b is a yellow-green pigment and will therefore absorb wavelengths best at yellow-green wavelengths, which are 525-560nm. 2. Chlorophyll a is a blue-green pigment and will therefore absorb wavelengths best at blue-green wavelengths, which are 450nm-500nm. 3. Xanthophyll is a yellow pigment and will therefore absorb wavelengths best at yellow wavelengths, which are 560nm-580nm. 4. Xanthophyll is a yellow pigment and will therefore absorb wavelengths best at yellow wavelengths, which are 560nm-580nm. 5. Carotene is an orange pigment and will therefore absorb wavelengths best at orange wavelengths, which are 600-700nm....
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This note was uploaded on 12/05/2011 for the course BIOSC 0050 taught by Professor Kaufmann during the Fall '06 term at Pittsburgh.

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