protein experimental protocol

protein experimental protocol - Sara ODonnell Protein...

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Sara O’Donnell Protein Purpose: In order to determine if various solutions contain protein, nine will be treated with two droppers full of Biuret’s reagent and monitored for color change from blue to violet or purple. The color change is evidence a protein is present and the copper ions in the Biuret’s reagent has reacted with the peptide bonds of the polypeptide chains. Scientific Hypothesis: Solutions containing proteins will change color after the addition of Biuret’s reagent from blue to purple. The more peptide bonds present, the deeper the purplish- violet color. Null Hypothesis: Solutions containing proteins will not change color after the addition of Biuret’s reagent. Predictions: 1. If Biuret’s reagent is added to water, then no color change will take place. 2. If Biuret’s reagent is added to pepsin, then the sample will change to a purplish color. 3. If Biuret’s reagent is added to albumin, then the sample will change to a purplish color. 4. If Biuret’s reagent is added to glycine, then the sample will change to a pink color.
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protein experimental protocol - Sara ODonnell Protein...

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