April 6 - miRNA - Therapeutic micro RNA Delivery Suppresses...

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Therapeutic micro RNA Delivery Therapeutic micro RNA Delivery Suppresses Tumorigenesis in a Suppresses Tumorigenesis in a Murine Liver Cancer Model Murine Liver Cancer Model Kota et al 2009 Kota et al 2009 Presenter: Jason Campbell
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MicroRNAs MicroRNAs ssDNA about 19-25 nucleotides Primary transcripts of miRNAs contain the hairpin structures of pre-miRNAs, 5’ Caps, and PolyA tails (Kim 2005)
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miRNA Processing miRNA Processing (Kim 2005)
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miRNA Processing miRNA Processing (Kim 2005)
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Results Results What? Which? Where? How? Which?
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miRNA Expression miRNA Expression mi-RNA to influence gene expression (Chang et al 2008) B cell lymphoma models Forced expression of these miRNAs Will this strategy work for other types of cancer?
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Chang et al 2008
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Myc-Induced Liver Tumor Model Myc-Induced Liver Tumor Model Bi-transgenic animal – a cross of two transgenic mice MYC transgene Transgene encoding a transactivating protein driven by a liver promoter Controlled by a tetracycline regulatory system
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Felsher and Bishop 1999
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Beer et al 2004
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The Search for miRNAs The Search for miRNAs Look for miRNAs at reduced levels in tumors Kota et al 2009
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Kota et al 2009 Are miR-26a results similar in human physiology?
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Enforce miRNA Expression Enforce miRNA Expression Murine Stem Cell Virus derived retroviral construct in HepG2 cells - miR-18a - miR-34a - miR-26a http://www.le.ac.uk/ge/genie/vgec/images/cellcycle.png
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Northern Blots. . .
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This note was uploaded on 12/06/2011 for the course BIOL 430 taught by Professor Dr.ianchin-sang during the Winter '10 term at Queens University.

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April 6 - miRNA - Therapeutic micro RNA Delivery Suppresses...

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