BIOL 430 2010 lecture 7 handout

BIOL 430 2010 lecture 7 handout - BIOL 430 Lecture BIOL 430...

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BIOL 430 Lecture 7 BIOL 430 Lecture 7 January 26 th 2010 How do we identify genes involved in development? Making transgenic animals Mosaic analysis BIOL 430 2010 Molecular Genetics of Development Dr. Ian Chin-Sang www.chin-sang.ca
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How do we Isolate Genes involved in Development? M t Mutagens Type of M t Type of DNA D Applications Mutagen Damage Chemical mutagenesis(i.e. EMS or Base Pair Change point mutation Saturation mutagenesis, efficient (1/1000) ENU N-ethylinitrosourea (ENU) ehtylmethanesulfonate (EMS) GC to AT efficient (1/1000), random, isolate any type of mutation lof or gof Transposons (i.e. P element or Tc1) DNA insertion not quite random, provides a “tag” for cloning isolate loss of cloning, isolate loss of function mutations as well as gain of function mutations Irradiation (X rays γ rays) Deletions and chromosomal rearrangements inefficient (1/10 000) random, usually isolate null mutations
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