BIOL 430 2010 lecture 16 handout 6 slides

BIOL 430 2010 lecture 16 handout 6 slides - 1 BIOL 430...

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Unformatted text preview: 1 BIOL 430 Lecture 16 February 16 th 2010 Caenorhabditis elegans BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) Eph RTK Signaling continued EFN-1 Ligand VAB-1 Receptor Activation? Is efn-1 in the vab-1 Pathway? BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) EFN-1 VAB-1 = vab-1(null) ??? X X efn-1 Functions in a vab-1 Dependent Pathway lity 100 Lethality Due to same Defects Seen in vab-1 animals BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) 30% % letha vab-1 (Null) efn-1 (Null) 80% 10 50 vab-1; efn-1 (Null) (Null) 84% VAB-1 and EFN-1 Signaling Between Neurons Is Required for Normal Epidermal Development X BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) Wild-type vab-1 or efn-1 mutant X Current work What genes function with vab-1 ? Genetics: Look for other Vab mutants Synthetic lethal Screens Molecular Biology and Biochemistry: Synthetic lethal Screens Suppressor Screens Identify Protein- Protein Interactions Yeast two hybrid screens Affinity chromatography Examples of other Morphogenesis Mutants wild type ju155 BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) vab-6 (ju164) ju165 2 Q. Why the variability or incomplete penetrance? A. Genetic redundancy. BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) Other genes that act in a similar process may compensate. Concept of Synthetic Lethality gene A Alive Mutation Phenotype gene B Alive gene A;gene B Dead BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) A B X essential developmental...
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This note was uploaded on 12/06/2011 for the course BIOL 430 taught by Professor Dr.ianchin-sang during the Winter '10 term at Queens University.

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BIOL 430 2010 lecture 16 handout 6 slides - 1 BIOL 430...

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