BIOL 430 2010 lecture 16 handout

BIOL 430 2010 lecture 16 handout - BIOL 430 BIOL 430...

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IOL 430 BIOL 430 Lecture 16 February 16 th 2010 Caenorhabditis elegans Eph RTK Signaling continued BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca)
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Is efn-1 in the vab-1 Pathway? EFN-1 Ligand VAB-1 Receptor Activation? EFN-1 AB-1 = vab 1(null) ??? ab (u) X X BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca)
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efn-1 Functions in a vab-1 ependent Pathway Dependent Pathway 100 y Lethality Due to same Defects Seen in ab nimals ethalit 50 vab-1 animals % l e 80% 84% 30% 10 BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) vab-1 (Null) efn-1 (Null) vab-1; efn-1 (Null) (Null)
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VAB-1 and EFN-1 Signaling Between Neurons Is equired for Normal Epidermal Development Required for Normal Epidermal Development X X ild- pe vab-1 or BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) Wild type efn-1 mutant
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Current work What genes function with vab-1 ? Genetics: ook for other Vab mutants Look for other Vab mutants Synthetic lethal Screens olecular Biology and Biochemistry: Suppressor Screens Molecular Biology and Biochemistry: Identify Protein- Protein Interactions east two hybrid screens Yeast two hybrid screens Affinity chromatography
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Examples of other Morphogenesis Mutants wild type ju155 ab (ju164) 165 BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) vab 6 (ju164) ju165
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. Why the variability Q. Why the variability or incomplete penetrance? A. Genetic redundancy. ther genes that act in Other genes that act in a similar process may compensate. BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca)
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Concept of Synthetic Lethality henotype gene A Alive Mutation Phenotype gene B Alive ene A;gene B ead gene A;gene B Dead eceptor A A B (A and B act redundantly or in a parallel pathway) Receptor A Receptor B X
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BIOL 430 2010 lecture 16 handout - BIOL 430 BIOL 430...

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