BIOL 430 2010 lecture 17 handout 6 slides

BIOL 430 2010 lecture 17 handout 6 slides - VAB-1 Eph RTK...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
1 BIOL 430 Lecture 17 February 17 th 2010 Caenorhabditis elegans Ephrin Signaling II BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) Genetic “tricks” and biochemical assays to find components in a signaling pathway VAB-1 Eph RTK May be a Receptor For a Stop or Repellent Cue Absence of VAB-1 Neurons overshoot their target (14% Genotype Phenotype BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) their target (14%) Too Much VAB-1 Neurons Stop too early (~100%) Constitutively Active Tyrosine Kinase MYR::VAB-1 Myristoylation Signal =MYR Partial loss of function mutations (hypomorph) in genes that encode A, B, BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) A B C C, or a gain-of-function mutation (hypermorph) in gene D may act as suppressors of activated VAB-1 D P P P VAB-1 VAB-1 Physically Interacts With at Least 8 Proteins Tyrosine Kinase Bait = Tyrosine Kinase Domain DAF-18/PTEN (5X) HIF-1 (5X) GLD-3/ BicC (3X) NCK-1 (1X)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

BIOL 430 2010 lecture 17 handout 6 slides - VAB-1 Eph RTK...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online