BIOL 430 2010 lecture 17 handout

BIOL 430 2010 lecture 17 handout - BIOL 430 Lecture 17...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: BIOL 430 Lecture 17 February 17 th 2010 Caenorhabditis elegans Ephrin Signaling II Genetic tricks and biochemical assays to find components in signaling pathway a signaling pathway BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) VAB-1 Eph RTK May be a Receptor or a Stop or Repellent Cue For a Stop or Repellent Cue enotype henotype Absence eurons overshoot Genotype Phenotype of VAB-1 Neurons overshoot their target (14%) Too Much VAB-1 Neurons Stop o early ( 100%) too early (~100%) BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) MYR::VAB-1 Myristoylation Signal =MYR Constitutively Active Tyrosine Kinase artial loss of function utations Partial loss of function mutations (hypomorph) in genes that encode A, B, C, or a gain-of-function mutation A (hypermorph) in gene D may act as suppressors of activated VAB-1 P P B C D P BIOL 430 Molecular Genetics of Development 2010 Dr. Ian Chin-Sang (www.chin-sang.ca) VAB-1 Physically Interacts With at Least 8 Proteins VAB-1 Bait = Tyrosine Kinase Domain AF 8/PTEN (5X) DAF-18/PTEN (5X) HIF-1 (5X) GLD-3/ BicC (3X) Tyrosine Kinase NCK-1 (1X) AP- /RAS GAP (1X)...
View Full Document

Page1 / 17

BIOL 430 2010 lecture 17 handout - BIOL 430 Lecture 17...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online