BIOL430 - presentation

BIOL430 - presentation - Adam Meets Eph An adam substrate...

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Adam Meets Eph: An adam substrate recognition module acts as a molecular switch for ephrin cleavage in trans Kristen Nemcko
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Outline Introduction to the Eph/ephrin family Introduction to ADAM Cell Adhesion and Repulsion Results Discussion Related Research
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Eph and ephrins Largest family of receptor tyrosine kinases (RTKs) Ligands = ephrins Cell-cell communication Spatial patterning and cell positioning Cell shape Cell migration 2 subclasses of Ephs and ephrins: A and B
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ADAM multi-domain transmembrane proteins Consists of: extracellular protease Disintegrin domain Cysteine-rich and EGF-like domains Involved in developmental events: (White, 2003) Myogenesis Neural crest migration Key regulators of the cell surface
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Cell Adhesion and Repulsion Ephrin/Eph interactions lead to bi- directional signaling and cell-cell repulsion Interacting Ephs and ephrins form heterotetramers (Himanen et. al, 2001) Cell repulsion occurs when tethers are broken
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Janes et al. (2005) This paper looked at: What are the interacting protein components? How do they interact with one another? Main components to the paper: Interactions Structure of ADAM10 Mechanisms of Ephrin Shedding
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Results - Interactions 1) What are the interactions between the molecules? Does ADAM10 associate with EphA3 or its ligand, ephrin-A5 on the cell surface? Transfection of HEK293 cells with EphA3 Coimmunoprecipitation of cell lysates with ADAM10 and EphA3 Western Blots
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Results
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Results 2) When does ADAM10 cleave ephrin-A5? Exposure to ephrin-A5, cleavage was seen Elevation of this process in cell cultures exposed to preclustered compared to non-clustered Fig 1C.
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Results
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This note was uploaded on 12/06/2011 for the course BIOL 430 taught by Professor Dr.ianchin-sang during the Winter '10 term at Queens University.

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BIOL430 - presentation - Adam Meets Eph An adam substrate...

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