Class Set of Slides (Somatic Sex Reprogramming)

Class Set of Slides (Somatic Sex Reprogramming) - Somatic...

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Unformatted text preview: Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 Ablation Jovian W., Jamey G. & Aliya S. Outline Introduction Gonad & Cellular Differentiation Transcription Factors Signaling Pathways Methods/Results Reprogramming of Granulosa to Sertoli Like Cells Testosterone Producing Leydig like Cell by loss of FOXL2 Discussion Insert Subtopics Conclusion What defines a male? What defines a female? http://graphics.cs.cmu.edu/courses/15- 463/2004_fall/www/handins/eryu/final/ http://graphics.cs.cmu.edu/courses/15- 463/2004_fall/www/handins/eryu/final/ What Infleunces Sex Determination? Sex Chromosomes XX : Female XY : Male Genetic Cascade Gene expression key determinant Transcription factors X versus Y influences Hormones Formation of secondary characteristics Males Testosterone Follicular Growth Factor 4 Prostaglandin Females Estrogen Progesterone Environment Male Development SRY switches testes development cascade ON SRY in combination with FGF9 and PTGSD induce upregulation of SOX9 SOX9 induces male gondal cell differentiation: Sertoli Cell Leydig Cells Mature male cells never enter apoptosis Female Development Wnt4 and RSPO1 are expressed activating the female development pathway Stabilization of -catenin Ectopic expression of -catenin (XY females) Stabilized -catenin trigger male pathway repressors FOXL2, DAX1 (SF1), ESR1/2 Repressors bind to TESCO inhibiting SOX9 production Formation of ovary and related cells: Theca Granulosa Undifferentiate d Gonads Wnt4 RSPO1 SRY -catenin SOX9 Ovar y Teste s FOXL2 DAX1 (SF1 ) Gonad Cellular Development Cell fate is determined once gender correct TFs are allowed to be expressed Granulosa Theca Leydig Sertoli Mutations ctopic duplication of SOX 9 in a fetus can lead to XX males nt4 and RSPO1 Loss of function mutations Partial male sex reversal of XX gonads AX1 Overexpression Sex-reversal in XY males lobal deletion of FOXL2 during development Ovary dysgenesis Infertility Activation of some testis specific genes Purpose of the Study Determine what happens when mutations arise that prevent the repression of SOX9 in ADULT female mice: Changes to sex differentiation pathway Loss of FOXL 2 and ESR1/2 receptor Ovary plasticity Reprogramming of cell fates The Problem Are mutations leading to the formation of leydig and sertoli like...
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This note was uploaded on 12/06/2011 for the course BIOL 430 taught by Professor Dr.ianchin-sang during the Winter '10 term at Queens University.

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Class Set of Slides (Somatic Sex Reprogramming) - Somatic...

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