Induced Pluripotent Stem Cells Seminar (Kilroy and Gill)

Induced Pluripotent Stem Cells Seminar (Kilroy and Gill) -...

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Induced Pluripotent Stem Cells Julia Gill http://www.ibt.tamhsc.edu/labs/ccscb/
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What is an Embryonic Stem Cell? Definition: Cells derived from the inner cell mass of a mammalian blastocyst (Wolpert et al. 2007 and Takahashi et al. 2007) Can be indefinitely maintained in culture (Wolpert et al. 2007 and Takahashi et al. 2007) http://www.csa.com/discoveryguides/stemcell/image luri.jpg
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What is Pluripotency? Definition (de Souza 2010) Cells that can differentiate into any cell type Are characterized by specific markers of pluripotency (Yamanaka 2007)
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What are iPS cells? First developed by Shinya Yamanaka and Kazutoshi Takahashi in 2006 using mice Nature technique of the year 2009 Lasker Basic Medical Research Award 2009 Description: http://repairstemcell.files.wordpress.com/2009/0 3/shinya-yamanaka-dr-skin-stem-cell.jpg
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Transcription Factors Associated with Pluripotency Oct3/4 Important in differentiation, especially neural and cardiac Sox2 Forms heterodimers with Oct3/4 for gene regulation Klf4 http://spin.niddk.nih.gov/clore/Structures/Oct- SOX/pict.html (Yamanaka 2007)
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More Transcription Factors Associated with Pluripotency c-Myc (Yamanaka 2007) Is important in modifying chromatin structure Loosened chromatin structure occurs at promoters for ES cell genes NANOG (Vallier et al. 2009) - Is an additional transcription factor that helps to maintain pluripotency Figure 5, Vallier et al. 2009.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors Authors: Kazutoshi Takahashi, Koji Tanabe, Mari Ohnuki, Megumi Narita, Tomoko Ichisaka, Kiichiro Tomoda, Shinya Yamanaka Journal: Cell Date: November 30, 2007 http://www.zoology.ubc.ca/~schluter/reprints/mille
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Introduction Purpose “[They] sought to generate iPS cells from
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This note was uploaded on 12/06/2011 for the course BIOL 430 taught by Professor Dr.ianchin-sang during the Winter '10 term at Queens University.

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Induced Pluripotent Stem Cells Seminar (Kilroy and Gill) -...

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