Regulation of the Transition from Gastrulation to Neurulation2

Regulation of the Transition from Gastrulation to Neurulation2

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Regulation of the Transition from Gastrulation to Neurulation by the Transcription Factor Churchill Miles Jackson, Thomas VanZuiden, Justin Wood
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Guojun Sheng, Mario dos Reis, and Claudio D. Stern* November, 2003 http://www.cell.com/archive?year=2003
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Outline Introduction To Churchill Study Organisms, Gastrulation, Neurulation Results/Discussion: Role of FGF + Sip-1 Repression of mesoderm markers Ingression through primitive streak + Neural induction Conclusions
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Churchill Zinc Finger protein named for the famous gesture of Sir Winston Churchill 112 aa protein containing 2 atypical C4 zinc fingers Unusual 23 aa and http://tametheblackdog.wordpress.com/2009/04/29/introduction/
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Churchill Expressed prior to mid-blastula transition specifically prospective neural plate Figure 1 from Sheng et al. ( 2003)
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Churchill Unique 23 – 24 aa spacing conserved in Chick, Fugu, Zebrafish, Xenopus, Rat, Mouse, and Human Each species only 1 gene with these characteristics Figure 1 from Sheng et al. ( 2003)
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Zinc Fingers Major structural motifs involved in protein-nucleic acid interactions Have many roles: (Pavletich and Pabo, 1991) Transcription factors Gal 4
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Chick Embryo as a Study Model Chick embryos provide an  ideal system because(Brookes  et al  1999): § Relatively Inexpensive § Rapid development § In Vivo analysis is simple  compared to viviparous  animals § Natural microenvironment  occurs in the egg Legend : AO  = Area Opaca AP  = Area Pellucida Arrows  = rapidly  dividing epiblast  cells P   = Posterior region  of embryo where  gastrulation begins Image from :  http://www.uoguelph.ca/zoolo gy/devobio/210labs/chickgastr ula.htm
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Gastrulation Rapid rearrangement of cells to form the  gastrula Begins the formation of a body plan  Defined by : The endoderm and mesoderm movement from the outside of the  embryo to the inside… involves cell migration and invagination Endoderm invaginates the blastula to form the gut after  mesenchymal (mesoderm) cells have entered the blastocoel During this stage the notochord is formed and lies beneath the  future nervous system This paper addresses 
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Regulation of the Transition from Gastrulation to Neurulation2

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