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Unformatted text preview: to apical enrichment in the ectodermal cells. wingless: wingless is a secreted signaling molecule responsible for segment polarity. the wild type phenotype is denticle belts with expanses of naked cuticle. It is part of the wnt gene family. Deletion mapping: This method using deletions to determine specific areas of genes. The experimenters used deletion mapping to determine the wingless localization elements as seen in the figure below. Western blots: separates proteins by length of polypeptide using gel electrophoresis Inducible expression: In this study heat shock was used to induce expression. Rescuing activity: Mating of null mutants with different transgenics can determine what characteristics of the transgenic are required for rescuing. In this study apical localization of wingless transcripts was required for appropriate signaling. Great resource: http://www.stanford.edu/~rnusse/wntwindow.html...
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- Winter '10
- DNA, wingless localization elements