lecturenoteschap4-10-1slide

lecturenoteschap4-10-1slide - BCMB 3100: Partial notes...

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BCMB 3100: Partial notes h t 4 t 1) Chapter 4 (Part 1) Diversity of proteins 3D structure of proteins Fibrous vs globular proteins Conformation vs configuration ° ° 3° nd 4° ructure 1 , 2 , 3 and 4 structure Peptide groups in polypeptide vs angles, Ramachandran plot Xray crystallography & NMR 1 helix vs -sheet
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Diversity of proteins ________________ - study of large sets of proteins, such as the entire complement of proteins produced by a cell E. coli has about _________ different olypeptides (average size 300 amino acids, polypeptides (average size 300 amino acids, M r 33,000) ruit fly ( rosophila melanogaster about • Fruit fly ( Drosophila melanogaster ) about 16,000, humans, other mammals about 0 000 different polypeptides 2 40,000 different polypeptides
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E. coli proteins on 2D gel electrophoresis Page 85 E. coli expresses ~4000 roteins p 3
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3D STRUCTURE OF PROTEINS wo classes of proteins: Two classes of proteins: ________________: water insoluble, static, "tough", extended, provide mechanical support ( -keratin, collagen) _______________: compact, "spherical", sually: hydrophobic interior & hydrophilic usually: hydrophobic interior & hydrophilic exterior enzymes 4
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BCMB 3100: Partial notes for Chapter 4 Diversity of proteins 3D structure of proteins Fibrous vs globular proteins Conformation vs configuration ° ° 3° nd 4° ructure 1 , 2 , 3 and 4 structure Peptide groups in polypeptide vs angles, Ramachandran plot Xray crystallography & NMR 5 helix vs -sheet
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The biological activity of a protein depends on its conformation _______________: spatial arrangement of substituent groups that are free to assume different positions in space, without breaking any bonds, ecause of the freedom of bond rotation because of the freedom of bond rotation The number of potential conformations of a protein is _____________. Under physiological conditions the protein assumes a single stable shape: native nformation conformation ________________: a spatial arrangement of atoms at can not be changed without breaking covalent 6 that can not be changed without breaking covalent bonds
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Levels of Protein Structure __________________: the covalent backbone of a polymer e residue y sidue conformation of ___________________: the residue-by-residue conformation of the backbone of a polymer : the 3D conformation of a polymer in its ___________________ py native folded state ___________________: the 3D structure of a multisubunit, particularly the manner in which the subunits fit together Supersecondary structure: clusters of secondary structure (e.g.   ) Domain: a distinct structural unit of a polypeptide; domains ave separate functions and may fold as independent, compact 7 have separate functions and may fold as independent, compact units
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Fig. 4.1 Levels of protein structure 8
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BCMB 3100: Partial notes for Chapter 4 Diversity of proteins D structure of proteins 3D structure of proteins Fibrous vs globular proteins Conformation vs configuration 1°, 2°, 3° and 4° structure Peptide groups in polypeptide s ngles Ramachandran plot vs angles, Ramachandran plot Xray crystallography & NMR 9 helix vs -sheet
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lecturenoteschap4-10-1slide - BCMB 3100: Partial notes...

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