lecturenoteschap5-10-2slides

lecturenoteschap5-10-2slides - 1 BCMB 3100 Chapter 5...

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Unformatted text preview: 1 BCMB 3100 Chapter 5 Lecture Six Classes (IUBMB) Six Classes (IUBMB) Kinetics Michaelis-Menten Equation Vo, Km, Vmax, Kcat Lineweaver-Burk Plot Six major groups of enzymes ( see pages 130-132 ) _________________ : (oxidation-reduction reactions) (group transfer reactions _________________ : (group transfer reactions) _________________ : (hydrolysis) ________________ : (nonhydrolytic and nonoxidative lysis double bond) (i i ti ________________ : (isomerization) ______________ : (ligation reaction requiring energy from NTP (nucleoside triphosphate) 2 1. Oxidoreductases ( dehydrogenases ) Catalyze oxidation-reduction reactions Oxidation can be identified as: * Addition of oxygen See also pg 199 (Box 7.2) and pg 200 Fig. 7.9 Other examples: pg 336, etc. Addition of oxygen * Removal of hydrogen * Increase in valency of metal ion (e.g. Fe +2 Fe +3 ) The Oxidation States of Carbon Most reduced CH 3-CH 3 Alkane (ethane) CH 3-HC 2 OH Alcohol (ethanol) CH 3-COH Aldehyde (acetaldehyde) CH 3-COOH Carboxylic acid (acetic acid) CO 2 Carbon dioxide Most oxidized Each of the arrows indicates an oxidation reaction. All except the last reaction are oxidations brought about by dehydrogenation. 3 2. Transferases Catalyze group transfer reactions 3. Hydrolases Catalyze hydrolysis reactions where water is the acceptor of the transferred group 4 4. Lyases Catalyze lysis of a substrate, generating a double bond in a nonhydrolytic nonoxidative double bond in a nonhydrolytic, nonoxidative elimination (i.e. nonhydrolytic & nonoxidative lysis that yields a double bond) 5. Isomerases Catalyze isomerization reactions 5 6. Ligases ( synthetases ) Catalyze ligation, or joining of two substrates Require chemical energy (e.g. ATP) Challenge of the Weekend, September 4, 2009 Find a minimum of five examples of enzymes and their reactions for each of the 6 classes of enzymes. (You should be able to find all or most of these in your book) Label an individual page with one of each of the names of the 6 classes of enzymes. On each page for that particular class of enzymes, give a list of at least 5 full reactions, including substrate(s), products and enzyme name. A l t d i t ill b i ith t t l f 30 ti A completed assignment will be six pages with a total of 30 reactions. For credit, these must be turned in before the start of Breakout Session on Tuesday, September 7. 6 BCMB 3100 - Chapter 5 Lecture Enzymes Six Classes (IUBMB) Kinetics Michaelis-Menten Equation Vo, Km, Vmax, Kcat Lineweaver-Burk Plot Principles of Kinetics (for non-enzyme-catalyzed reactions) (1) first -order reaction k S P [P] = v = k[S] rate equation t v = velocity, P = product, S = substrate, k = rate constant (s-1 ) 7 Principles of Kinetics (for non-enzyme-catalyzed reactions) (2) second-order reaction (bimolecular) S 1 + S 2 P 1 + P 2 v = k[S 1 ] 1 [S 2 ] 1 S 1 + S 2 P 1 + P 2 v k[S 1 ] [S 2 ] k = rate constant M...
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lecturenoteschap5-10-2slides - 1 BCMB 3100 Chapter 5...

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