lecturenoteschap5-10-4slides

lecturenoteschap5-10-4slides - BCMB 3100 Chapter 5 Lecture...

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1 BCMB 3100 – Chapter 5 Lecture Six Classes (IUBMB) Kinetics Michaeli Menten Equation Michaelis-Menten Equation Vo, Km, Vmax, Kcat Lineweaver-Burk Plot Six major groups of enzymes ( see pages 130-132 ) _________________ : (oxidation-reduction reactions) _________________ : (group transfer reactions) _________________ : (hydrolysis) ________________ : (nonhydrolytic and nonoxidative lysis double bond) ________________ : (isomerization) ______________ : (ligation reaction requiring energy from NTP (nucleoside triphosphate) 1. Oxidoreductases ( dehydrogenases ) Catalyze oxidation-reduction reactions See also pg 199 (Box 7.2) and pg 200 Fig. 7.9 Other examples: pg 336, etc. Oxidation can be identified as: * Addition of oxygen * Removal of hydrogen * Increase in valency of metal ion (e.g. Fe +2 Fe +3 ) The Oxidation States of Carbon Most reduced CH 3 -CH 3 Alkane (ethane) CH 3 -HC 2 OH Alcohol (ethanol) CH 3 -COH Aldehyde (acetaldehyde) CH 3 -COOH Carboxylic acid (acetic acid) CO 2 Carbon dioxide Most oxidized Each of the arrows indicates an oxidation reaction. All except the last reaction are oxidations brought about by dehydrogenation.
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2 2. Transferases • Catalyze group transfer reactions 3. Hydrolases • Catalyze hydrolysis reactions where water is the acceptor of the transferred group 4. Lyases • Catalyze lysis of a substrate, generating a double bond in a nonhydrolytic, nonoxidative elimination (i.e. nonhydrolytic & nonoxidative lysis that yields a double bond) 5. Isomerases • Catalyze isomerization reactions
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3 6. Ligases ( synthetases ) • Catalyze ligation, or joining of two substrates • Require chemical energy (e.g. ATP) Challenge of the Weekend, September 4, 2009 Find a minimum of five examples of enzymes and their reactions for each of the 6 classes of enzymes. (You should be able to find all or most of these in your book) Label an individual page with one of each of the names of the 6 classes of enzymes. On each page for that particular class of enzymes, give a list of at least 5 full reactions, including substrate(s), products and enzyme name. A completed assignment will be six pages with a total of 30 reactions. For credit, these must be turned in before the start of Breakout Session on Tuesday, September 7. BCMB 3100 - Chapter 5 Lecture Enzymes Six Classes (IUBMB) Kinetics Michaelis-Menten Equation Vo, Km, Vmax, Kcat Lineweaver-Burk Plot Principles of Kinetics (for non-enzyme-catalyzed reactions) (1) first -order reaction k S P [P] = v = k[S] rate equation t v = velocity, P = product, S = substrate, k = rate constant (s -1 )
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4 Principles of Kinetics (for non-enzyme-catalyzed reactions) (2) second-order reaction (bimolecular) S 1 + S 2 P 1 + P 2 v = k[S 1 ] 1 [S 2 ] 1 k = rate constant M -1 s -1 pseudo first-order reaction If concentration of a reactant is so high that it remains constant during reaction = zero-order v = k[S 1 ] 1 [S2] = k[S 1 ] 1 Important for enzyme assays Emil Fischer 1894 ____________ model for enzyme:substrate interactions Substrate Product Enzyme ES complex Enzyme Enzyme Kinetics Enzyme-substrate complex ( ES ) - complex formed when specific substrates fit into the enzyme active site E + S ES E + P
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This note was uploaded on 12/03/2011 for the course CHEM 3100 taught by Professor Dervartanian during the Fall '09 term at UGA.

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lecturenoteschap5-10-4slides - BCMB 3100 Chapter 5 Lecture...

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