Fig 20.1•Meselson & Stahl, 1958•Semiconservative DNA replication•Each strand of DNA acts as a template for synthesis of a new strand•Daughter DNA contains one parental and one newly synthesized strand
Enzymatic Synthesis of DNAArthur Kornberg (1955-58) discovered an enzyme that synthesized DNAExperimental Strategy1) dNTPs as precursors of DNA2) sensitive assay to detect newly synthesized DNA; radioactive dNTPs & acid precipitation of DNA3) When animal cell extracts proved unsuccesful they turned to E. coliE colidivides fast (every 20 minutes) and largeE. coli divides fast (every 20 minutes) and large quantities of cells can be isolated
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