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lecturenoteschap20-10-1slide

lecturenoteschap20-10-1slide - BCMB 3100 Chapter 20 DNA...

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BCMB 3100 - Chapter 20 DNA Replication Semi-conservative DNA replication DNA polymerase DNA replication Replication fork; Okazaki fragments Sanger method for DNA sequencing DNA repair PCR
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Fig 20.1 Meselson & Stahl, 1958 Semiconservative DNA replication Each strand of DNA acts as a template for synthesis of a new strand Daughter DNA contains one parental and one newly synthesized strand
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Enzymatic Synthesis of DNA Arthur Kornberg (1955-58) discovered an enzyme that synthesized DNA Experimental Strategy 1) dNTPs as precursors of DNA 2) sensitive assay to detect newly synthesized DNA; radioactive dNTPs & acid precipitation of DNA 3) When animal cell extracts proved unsuccesful they turned to E. coli E coli divides fast (every 20 minutes) and large E. coli divides fast (every 20 minutes) and large quantities of cells can be isolated
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