cheng - The Time Scale of the Catalytic Loop Motion in...

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The Time Scale of the Catalytic Loop Motion in Triosephosphate Isomerase S. Rozovsky; A. E. McDermott; J. Mol. Biol. (2001) 310 , 259-270 Javelin Cheng September 21, 2005
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Outline Background Classical Enzymology Function of TIM Structure Motivation Methods Results Population control Temperature dependence Conclusion Discussion
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Classical Enzymology
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Function First discovered by Meyerhof and Kiessling in 1935 Catalyzes the conversion of DHAP into GAP via proton transfer Thermodynamically very efficient, k cat = 9x10 3 s -1
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Structure Two conformers: open and closed Loop 6 moves substantially Proposed rate limiting step is the loss of GAP product from the enzyme
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Motivation X-ray crystallography indicates two structures but do not provide any information about kinetics Characterize conformational dynamics and understand the relation between rate and enzyme optimization SSNMR can more accurately provide information about motions on timescales of seconds to pico-seconds
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cheng - The Time Scale of the Catalytic Loop Motion in...

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