FINAL BIO LAB REPORT-1209

FINAL BIO LAB REPORT-1209 - Landry 1 The Effect of...

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Landry The Effect of Temperature on Chlamydomonas reinhardtii Growth Michael Landry Section 24 May 5, 2011 1
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Landry Abstract During this experiment, tests are performed to determine whether or not various temperatures change the population density of the green algae, Chlamydomonas reinhardtii. To correctly perform Equal amounts of C. reinhardtii were placed in one of three environments (4, 22, and 30º C), and their growth was monitored for 14 days. The C. reinhardtii at 22º C had the greatest increase in population density after each week. The algae at 4º C had the least increase in population density after each week. After comparing the population densities, it is concluded that the alternative hypothesis is rejected for the populations grown at 30º C because the readings from day 14 do not show a significant difference in growth between 22º C and 30º C. However, the alternative hypothesis is accepted for 4º C. In extreme temperatures, growth of C. reinhardtii is restricted because the flagella are inhibited, decreasing motility. Prolonged exposure to extreme temperatures results in loss of flagella and, eventually, cell death (Huang, Rifkin, and Luck, 1977). The results from day 14 signify that 30º C is not an extreme enough temperature to inhibit growth in the populations of C. reinhardtii. Theoretical Background Chlamydomonas reinhardtii is a unicellular green algae that is capable of self- movement and has eyespots that detect light. It is found in fresh water as well as in soil. C. reinhardtii is a common choice for studies involving biology and ecology because of its short life span, ability to reproduce both sexually and asexually, and ability to be both a heterotroph as well as a facultative autotroph (“ Chlamydomonas reinhardtii ,” 2006). The objective of this experiment was to find out what effect the temperatures, 4 º C, 22 º C, and 30 º C, have on the population density of Chlamydomonas reinhardtii over the course of 14 days. In accordance to a similar study, an extreme cold or hot temperature lowers the production rate of C. reinhardtii (Spreitzer, 1988). These results show that a population of C. reinhardtii grows the quickest in a lake at a point below the surface that is not on the immediate surface or in the depths. The deeper or shallower the depth, the slower the rate of growth is going to be because these depths receive the most and least amount of sunlight, reaching the highest and lowest temperatures (Janssen, 1999). At
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FINAL BIO LAB REPORT-1209 - Landry 1 The Effect of...

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