Microscope Cheat Sheet

Microscope Cheat Sheet - Name: __________________________...

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Unformatted text preview: Name: __________________________ Date: ___________ P____ S____ Metric Prefixes and Microscopic Measurement x Prefix tera ­ giga ­ mega ­ kilo ­ hecto ­ deka ­ unit deci ­ centi ­ milli ­ micro ­ nano ­ pico ­ Abbreviation T G M k h da l, g, m d c m µ n p Value 1,000,000,000,000 1,000,000,000 1,000,000 1,000 100 10 1 .1 .01 .001 .000001 .000000001 .000000000001 Value Trillion Billion Million Thousand Hundred Ten One Tenth Hundredth Thousandth Millionth Billionth Trillionth Exp Form 1012 109 106 103 102 101 100 10 ­1 10 ­2 10 ­3 10 ­6 10 ­9 10 ­12 Saying The / giant martian kissed honest decent ones. Dancing can make many nights passionate. *Moving down the chart, you would multiply and the decimal would move to the right, increasing the number. *Moving up the chart, you would divide and the decimal would move to the left decreasing the number. 10 ­6 10 ­5 10 ­4 10 ­3 10 ­2 10 ­1 10 ­0 101 102 103 104 105 106 µ m c d base da h k M Unit (m, l, g) Magnification & Field of View As the magnification increases, the field of view decreases by the same proportion. Ocular Power Objective Magnification FOV Field of View measurement 10x Low 4x mag ⇑10x FOV⇓10x High 40x 40x 4000µm 10x 400x 400µm Estimating the Microscope’s Field of View Diameter 2. Be sure your ruler is horizontal across the field. 1. Set your ruler so that you line one edge of a major division on one edge of the field 3. Estimate the diameter field diameter = 5.2mm Finding the ACTUAL size of cell: One cell 1. Determine the diameter of the field of view. Assume you are observing the above cells through the high power (400x) of your microscope. 2. Determine the measurement of your FOV. Assume for the sake of this example that the FOV is 400µm. 3. Approximate (as accurately as possible) how many cells across the field of view. In the graphic given, approximately 1.2 cells fit across the FOV. 4. Divide the diameter of the FOV by the number of cells that fit across. 400µm/1.2 = 333.33µm per cell. Finding the scale of your drawing of the cell: We can use scale to compare linear measures. A scale of 1:1 implies that the drawing is identical in size to the object. A scale of 1:2 indicates that the drawing is ½ the size of the object. A scale of 2:1 suggests that the drawing is two times larger than the actual object. The first number is the number of units in the drawing and the second number is the equivalent number of units in the real life object. After you complete your drawing of the cell you’ve measured, use “Finding the ACTUAL size of the cell”, measure the length of your drawing of the cell. Measure in cm or mm. Steps: 1. measure the length of the cell drawing for example: 4cm 2. convert the length of your drawing to µm for example: 4cm x 10,000µm/cm = 40,000µm 3. Divide the drawing size by the actual cell size for example: 40,000µm/333.33µm = 120 4cm 4. Write your scale. The number you determined in #3 goes first followed by a colon to indicate a ratio, followed by a 1. for example: scale = 120:1 Note that the 120 represents # of units (µm) in the drawing and the 1 represents the # of units (µm) in the actual cell. If your drawing was of a larger object than what you drew like the microscope, you would have a 1 to indicate the # of units in the drawing and a larger number to indicate units in the microscope. ...
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