2 lecture - T7 DNA Pol crystal T7 DNA Pol active site -...

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B-form DNA has two grooves of unequal size-Why?
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Figure 4-3 Molecular Biology of the Cell (© Garland Science 2008)
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Figure 4-5b Molecular Biology of the Cell (© Garland Science 2008)
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Why are the two strands antiparallel? Only in the antiparallel orientation do the H bond donors and acceptors line up.
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Two detailed views of asymmetric attachment
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Figure 7-7 Molecular Biology of the Cell (© Garland Science 2008) Asymmetry in the plane of a base-pair gives rise to the major and minor grooves
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Figure 7-8 Molecular Biology of the Cell (© Garland Science 2008) Another way of portraying the greater molecular information content of the major vs minor grooves
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Figure 7-9 Molecular Biology of the Cell (© Garland Science 2008)
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The overall geometry of each base-pair is invariant. This is important for the Fdelity of replication, DNA repair and understanding how sequence information is accessed by DNA binding proteins.
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Zooming into the elongating DNA strand
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Figure 06-05
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Figure 06-06 Mechanism of DNA polymerization
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Unformatted text preview: T7 DNA Pol crystal T7 DNA Pol active site - stick T7 DNA Pol active site - surface Table 5-1 Molecular Biology of the Cell ( Garland Science 2008) Fidelity of DNA Replication: Mismatches perturb the active site of DNA polymerase and stall polymerization Figure 5-4 Molecular Biology of the Cell ( Garland Science 2008) Figure 5-9 Molecular Biology of the Cell ( Garland Science 2008) Why does DNA replication only occur 5->3? Consider the consequence for proofreading if replication was 3 to 5. Figure 06-06 Some nucleotides can be incorporated by DNA polymerases without triggering recognition of altered structure. H Figure 8-50a Molecular Biology of the Cell ( Garland Science 2008) Figure 8-50b Molecular Biology of the Cell ( Garland Science 2008) Figure 8-50c Molecular Biology of the Cell ( Garland Science 2008) Sanger DNA sequencing Figure 16-39 How we do Sanger sequencing now OH What is this?...
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This note was uploaded on 12/05/2011 for the course BIO 344 taught by Professor Herrin during the Spring '08 term at University of Texas at Austin.

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2 lecture - T7 DNA Pol crystal T7 DNA Pol active site -...

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