Jake HobbickMIC 201L Sec.9October 25, 2017Lab 3 DiscussionDiscussion:In part one of the lab, two non-synthetic and two synthetic culture tubes were preparedthen analyzed after one of each of the tubes were autoclaved.Theoretically due to two of the tubes being autoclaved and the other two being left out,the two autoclaved tubes would be absent of growth while the other two containedgrowth. Experimentally this did not occur, all four tubes were absent of growth. This ispossibly due to either the broths being incorrectly made or through the unlikely chancesof no bacteria contaminating the tubes. This may be fixed by intentionally introducingsome type of bacteria to the broth to ensure growth in the non-autoclaved tubes.Understanding autoclaving and sterilization techniques are very important in amicrobiology lab for preventing contamination and ensuring accurate results.In part two of the lab,Escherichia coli,Pseudomonas aeruginosa,Enterococcusfaecalis,andStaphylococcus aureus
You've reached the end of your free preview.
Want to read both pages?
Bacteria,Escherichia coli,Jake Hobbick,Sodium azide agar plate