MIC 201L Lab 3 Discussion.docx - Jake Hobbick MIC 201L Sec.9 Lab 3 Discussion Discussion In part one of the lab two non-synthetic and two synthetic

MIC 201L Lab 3 Discussion.docx - Jake Hobbick MIC 201L...

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Jake Hobbick MIC 201L Sec.9 October 25, 2017 Lab 3 Discussion Discussion: In part one of the lab, two non-synthetic and two synthetic culture tubes were prepared then analyzed after one of each of the tubes were autoclaved. Theoretically due to two of the tubes being autoclaved and the other two being left out, the two autoclaved tubes would be absent of growth while the other two contained growth. Experimentally this did not occur, all four tubes were absent of growth. This is possibly due to either the broths being incorrectly made or through the unlikely chances of no bacteria contaminating the tubes. This may be fixed by intentionally introducing some type of bacteria to the broth to ensure growth in the non-autoclaved tubes. Understanding autoclaving and sterilization techniques are very important in a microbiology lab for preventing contamination and ensuring accurate results. In part two of the lab, Escherichia coli , Pseudomonas aeruginosa , Enterococcus faecalis, and Staphylococcus aureus
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