335-11_lec2 - BIO 335 lecture (2) Action potentials...

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BIO 335 lecture (2) Action potentials continued. . • Review first two labs ( clickers ) • Cable properties of the axon - Size & the length constant - Myelination • This week’s lab: recording compound action potentials in a vertebrate nerve
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Extracellular recording of action potentials from earthworm giant fibers To differential amplifier Recording electrodes Stimulating electrodes
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S1 (+) S2 (–) R1 R2 The different phases ( a and b ) of this extracellular action potential signal are due to… A. …the S1 and S2 stimulating electrodes. B. …the R1 and R2 recording electrodes. C.…action potentials in different giant fibers. D.…axon depolarization (a) and hyperpolarization (b) Clicker question a b
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S1 (+) S2 (–) R1 R2 Q. Which electrode is connected to the inverting input of the amplifier? A. R1 B. R2 C.Not enough information to know Clicker question a b
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Q. How do signals i and ii most likely arise ? A. - from different stimulating electrodes. B. - from different recording electrodes. C. - from action potentials in different giant fibers. D. - from successive action potentials in the same giant Clicker question i ii
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Clicker question S1 S2 R 1 R 2 Q. Some groups observed monophasic action potential signals, as seen here. One possible cause is…. . A. …failure of one stimulating electrode B. …a break in a giant fiber, between R1 and R2 . C.…a break in a giant fiber, between S2 and R1 . D.…failure of one recording electrode.
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Clicker question Q. What is the most likely peak-peak amplitude ( ) of the potential change at the recording electrodes , with equipment set up as in lab?
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335-11_lec2 - BIO 335 lecture (2) Action potentials...

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