Session 1 [Equipment, Giant Axon]

Session 1 - BIO335 SESSION 1 LAB INSTRUCTIONS WEEK 1 INTRODUCTION TO EQUIPMENT SINGLE ACTION POTENTIALS IN GIANT AXONS Part 1 of the first session

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BIO335 SESSION 1 1 LAB INSTRUCTIONS WEEK 1: INTRODUCTION TO EQUIPMENT, & SINGLE ACTION POTENTIALS IN GIANT AXONS Part 1 of the first session will familiarize you with the electrophysiology equipment and computer programs that you will use throughout much of the course. It is important to be able to connect, use, and troubleshoot your set-up efficiently, so that you can spend more time on physiology than on electronics. You will learn how to : 1) Specify and deliver an electrical stimulus using a pulse generator 2) Record an electrical potential using a computer-based data acquisition system, 3) Display and analyse measurements of the stimuli using the computer system, 4) Print the data and save your work. Part 2 of the first session will introduce the first physiological experiment: extracellular recording of single action potentials from the giant axons in an annelid, the earthworm. You will learn how to : 1) Set up a pulse generator and stimulus isolator to deliver stimulus current pulses. 2) Use a virtual oscilloscope to detect and record action potentials, and to quantify timing and amplitude of action potentials in different axons. 3) Vary stimulus properties to discover corresponding threshold stimulus levels. 4) Estimate the conduction velocities and refractory periods for different axons. These instructions include: I. BACKGROUND: READ BEFORE LAB. The Background to Part I details the functions of the equipment that you will use: it is very important that you understand these before the lab session if you are to get anything done. II. EXERCISES: the procedures that you will follow during lab. Also includes queries that you should try to answer as you go; related questions may reappear in the. .. ....WORKSHEET (for parts 1 and 2 combined) of questions to be answered after lab. This will be distributed during the lab session. The worksheet plus printed screenshots of data will constitute your report for this first lab session. You are also asked to write on the whiteboard at the front of the lab some of calculated results, as your group obtains them.
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BIO335 SESSION 1 2 Overview – How electrophysiology experiments work Many experiments in the first part of this course follow essentially the same procedure: a preparation such as a nerve is stimulated at one location with a pulse of electric current, and any resulting changes in voltage are recorded from a different site, amplified, displayed and analysed. Because each episode of stimulus and neural response is complete in as few as ten milliseconds, the stimulus and recording must be tightly synchronized, and the data captured at high sampling frequency. Here is how this is done: - Stimuli are designed at the Pulse generator (Accupulser), which produces square voltage pulses with durations and intervals that you specify. - These pulses control the
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This note was uploaded on 12/06/2011 for the course BIO 335 taught by Professor Cabot during the Fall '08 term at SUNY Stony Brook.

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Session 1 - BIO335 SESSION 1 LAB INSTRUCTIONS WEEK 1 INTRODUCTION TO EQUIPMENT SINGLE ACTION POTENTIALS IN GIANT AXONS Part 1 of the first session

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