Lecture 19 - Qdot605 EGFR TM JM 11/9/11 7 EGF-Qdot E/TrkB...

Info iconThis preview shows pages 1–13. Sign up to view the full content.

View Full Document Right Arrow Icon
11/9/11 1 Signaling Over Time and Space 1. Why do NGF and EGF actions differ in neurons? 2. Why do the EGF receptor (proto-oncogene) and the EGFR oncogene have different effects in neurons?
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
11/9/11 2 Signaling Over Time and Space Differential signaling over time by NGF and EGF +NGF +EGF PC12 cells Erk activation Erk Erk MEK MEK
Background image of page 2
11/9/11 3 Sustained Erk kinase signaling Erk kinase
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
11/9/11 4 Receptor-mediated endocytosis via clathrin
Background image of page 4
11/9/11 5 Plasma Clathrin-coated Ruffle actin Early Endosome Macroendosome Rab5 Rab5 EEA1 Rabe Ank Multivesicular Bodies P R Ank Rab5 Rabe Rab5 Ank Rabe Rab5 Rab7 P P Pit Membrane Ank Rab5 Ank Rabe Rab5 P Late endosome/ Lysosome P-Erk P-Erk P-Erk X P-Erk P-Erk P-Erk P-Trk P-Trk P-Trk P-Trk P-EGFR P-EGFR EGFR X nucleus nucleus P-EGFR Endosomal Signaling Peripheral Nervous System Central Nervous System NGF responsive neuron NGF responsive neuron NGF producing neuron NGF producing Non-neuronal cell =TrkA =NGF ~1m
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
11/9/11 6 Tracking retrogradely transported endosomes . . . . . . Cell bodies Distal axons EGF-Qdot605 EGF EGFR/Trk
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
Background image of page 13
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Qdot605 EGFR TM JM 11/9/11 7 EGF-Qdot E/TrkB EGF-Qdot P-Erk two hours later look at cell body neurons expressing EGF/Trk chimera by viral vector Ultrastructure of Retrogradely Transported Endosomes 11/9/11 8 Cell body Axon EGF-Qdot/EGFR Trk and EGFR endosomes are retrograde transported Axon Cell body EGF-Qdot/E-TrkB 200 nm What are the molecular components of the signaling endosome? 11/9/11 9 EGF and EGFR co-localize in endosomes EGF-nGold EGFR 200 nm FRS2 ARMS M P 1 11/9/11 10 P P P P P P P P P P P Erks Nucleus = NGF = TrkA = actin = Rab5 = Rabankyrin = Rabenosyn = Pincher = Rac P Sustained signaling Neurotrophin/Trk P P P P P P P P P P = EGF = EGFR Erks Nucleus Transient signaling = Rab7 EGF/EGFR Subcellular Compartmentation of Signaling: Anchoring and Scaffolding 11/9/11 11 Cell Surface Endosome Rap B-Raf p14 Erk locale KSR MP1 11/9/11 12 A-kinase Anchoring Protein (AKAP) AKAPs localize kinases with specifc substrates 11/9/11 13...
View Full Document

This note was uploaded on 12/06/2011 for the course BIO 317 taught by Professor Simonhalegoua during the Spring '08 term at SUNY Stony Brook.

Page1 / 13

Lecture 19 - Qdot605 EGFR TM JM 11/9/11 7 EGF-Qdot E/TrkB...

This preview shows document pages 1 - 13. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online