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Chapter+17++Student+Overheads - Spectrophotometry Basic...

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Figure 17-4: Daniel C. Harris, Quantitative Chemical Analysis , 8 th edition. Basic Diagram of a Spectrophotometry Experiment Spectrophotometry
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Vibrational Transitions Electronic Transitions Nuclear Transitions Rotational Transitions Figure 17-2: Daniel C. Harris, Quantitative Chemical Analysis , 8 th edition. The Electromagentic Spectrum
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Figure 17-1: Daniel C. Harris, Quantitative Chemical Analysis , 8 th edition. Properties of Light
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Absorbance vs. Concentration -0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0 0.1 0.2 0.3 0.4 Concentration (M) % T vs. Concentration 0 20 40 60 80 100 120 0 0.1 0.2 0.3 0.4 0.5 Concentration (M) Absorbance vs. Transmittance % T Absorbance
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Spectrophotometry
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OH CH(CH 3 ) 2 C H 3 C Br S O O O CH(CH 3 ) 2 OH Br H 3 C O CH(CH 3 ) 2 C H 3 C Br SO 3 - CH(CH 3 ) 2 OH Br H 3 C λ max = ~ 420 nm Acidic Form (HIn) Basic Form (In - ) λ max = ~ 620 nm Bromothymol Blue Spectrophotometry
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0 0.1 0.2 0.3 0.4 0.5 0.6 300 400 500 600 700 800 Wavelength (nm) Absorbance λ max (HIn) = 420 nm λ max (In - ) = 620 nm Solution of bromothymol blue at pH 10.00.
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