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Chapter+25+Student+Overheads - Conducting Ion Exchange...

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Affinity Chromatography Figure 25-19: Daniel C. Harris, Quantitative Chemical Analysis , 8 th ed.
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Molecular Exclusion Chromatography glycerin
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Gel Stationary Phase Large Molecules Small Molecules Mobile Phase Flow Figure 25-14: Daniel C. Harris, Quantitative Chemical Analysis , 8 th ed. Molecular Exclusion Chromatography
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Molecular Exclusion Chromatography
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Molecular Exclusion Chromatography Figure 25-17: Daniel C. Harris, Quantitative Chemical Analysis , 8 th ed.
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Ion-Exchange Chromatography
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Ion Exchange Stationary Phase Amorphous particles of organic material Example: Polystyrene Divinylbenzene (1- 16%) is used to cross-link chains and impart more rigidity More cross-linking: smaller pores, longer equilibration time for solute between inside/outside of particle
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Unformatted text preview: Conducting Ion Exchange Chromatography CH 2 C O O-Na + CH 2 C O O-Na + CH 2 C O O-Na + CH 2 C O O-Na + Li + Li + Li + Li + Cl-Cl-Cl-Cl-MP CH 2 C O O-Li + CH 2 C O O-Li + CH 2 C O O-Li + CH 2 C O O-Li + Cl-Cl-Cl-Cl-Na + Na + Na + MP Initial Final Gradient Elution in Ion Exchange Chromatography + + + + + + + + + + + + + + + + + + + + + + + + Animation by Dan Riccio, fall 2007 GAAN student. Applications of Ion Exchange Ion Exchange Selectivity Selectivity coefficient, K Example: R-Na + + Li + R-Li + + Na + Ion-exchangers favor: Higher charge Smaller hydrated radius Increased Polarizability...
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This note was uploaded on 12/06/2011 for the course CHEM 241 taught by Professor Tiani during the Fall '08 term at UNC.

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Chapter+25+Student+Overheads - Conducting Ion Exchange...

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