Study Guide for Exam 2
Study Guide- Exam 2
(covers Classes 2.1 through 2.6)
Class 2.1---02/08/07: The Genetic Code
Know the following terms:
Codon, anticodon, synonym, third-base degeneracy, wobble hypothesis, proofreading (relative to
synthethase charging), suppressor tRNAs, free ribosomes, membrane-bound ribosomes, post-
translational translocation, co-translational translocation, signal sequence, signal recognition
particle (SRP), SRP receptor, 11S ribonucleoprotein complex, 7S RNA, translocon.
Understand the Following Concepts:
1. Be able to use the codon dictionary to translate a strand of mRNA.
2. Be able to pick out the wobble base in a codon and an anticodon.
3. Be able to recognize some of the more common modified bases found in tRNAs.
4. Know which bases Inosine, queuosine, and 2-thiouridine can base pair with.
5. Understand the relationship between the number of codons, tRNAs, and aminoacyl-tRNA
6. Understand the mechanism of how aminoacyl-tRNA synthetases are charged.
7. Understand how a suppressor tRNA works to suppress a missense or nonsense mutation.
8. Know where and how protein synthesis takes place in different locations within cells.
9. Know the difference and understand the mechanisms for post-translational translocation and
co-translational translocation of proteins.
10. Understand the structure and role of the signal sequence in synthesizing proteins that are
membrane bound or secreted.
11. Know the structure of the SRP and the SRP receptor.
12. Understand the role of GTP in co-translational translocation.
13. Know the structure of the translocon.
14. Understand the general mechanisms by which proteins are imported into mitochondria and
Class 2.2---02/15/07: Transcription
Promoter, startpoint, terminator, transcription unit, pre-mRNA, upstream sequence, downstream
sequence, transcription bubble, closed promoter complex, open promoter complex, bridge, ,
holoenzyme, core enzyme, sigma factos, tight binding, abortive initiation, promoter clearance, up
promoter mutations, down promoter mutations, gyrase, topoisomerase I, antitermination,
intrinsic terminators, rho-dependent terminators, rut sites.
1. Be able to predict the sequence and orientation of an RNA transcribed
from a labeled
double-stranded DNA molecule.