Study Guide IV - Study Guide for the Final Exam Study Guide...

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Study Guide for the Final Exam Page 1 Study Guide- Final Exam Study Guide- Comprehensive Part - 28 questions will be taken from previous exams (14 multiple choice, 14 half short answers). - These questions may be reworded; DO NOT try to memorize old answers. - The way to study for the comprehensive part of final is to understand why original answer was correct, AND why the other choices were wrong. Study Guide- Part 4 (covers 4.1 through 4.6) Class 4.1---04/12/07: Exceptions to the Fundamentals of Molecular Biology Know the following terms: Ribozymes, Group I introns, Group II introns, L19 catalytic RNA, riboswitch, maturases, viroids, virusoids, hammerhead structures, RNA editing, deaminase enzymes, terminal uridyltransferase (TUTase), RNA ligase, guide RNAs, exteins, inteins. Understand the Following Concepts: 1. Know how a guanine nucleotide acts as a cofactor for the splicing of Group I introns. 2. Know the types of genes that contain Group I introns. 3. Know the mechanism for splicing or the 26S rRNA in Tetrahymena . 4. Know how the secondary structure of Group I introns leads to self splicing. 5. Know how the L19 RNA generated from a Group I intron can catalyze the addition of nucleotides onto small RNAs. 6. Understand the mechanism by which a ligand can activate a riboswitch. 7. Know that some Group I and II introns contain ORFs. Know what kinds of proteins these ORFs produce and how they are related to the mechanism of intron spreading and intron homing. 8. Understand how viroids and virusoids can be infectious. 9. Know how ribozymes can be engineered to be tools for silencing specific genes. 10. Know how RNA editing can produce different proteins without affecting the sequence of the gene. 11. Understand the mechanisms by which RNA editing can change the RNA sequence. 12. Know the composition of the RNA editing complex. 13. Know the 4 stages of RNA editing using a guide RNA. 14. Know the relationship between inteins, exteins, and protein splicing. Class 4.2---04/17/07: Chromosomes Terms:
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Study Guide for the Final Exam Page 2 Capsid, terminase enzyme, nucloid, free supercoiling, restrained supercoiling, metaphase chromosome, chromatin, euchromatin, constitutive heterochromatin, facultative heterochromatin, karyotype, giemsa staining, G-banding, lampbrush chromosomes, polytene chromosomes, centromere, C-banding, kenetochore, microtubule organizing center (MTOC), CEN fragment, CDE-1, CDE-II, CDE-III, telomere, telomerase complex. Concepts: 1. Know the two different ways that nucleic acids are packaged in viruses or phages. 2. Know how translocation and condensation are related to the stages of the packaging in viruses. 3. Know how bacterial chromosomes are packaged.
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Study Guide IV - Study Guide for the Final Exam Study Guide...

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