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Unformatted text preview: on the SDS gel shown below. What can you conclude about your protein? 10 kD 8 kD 6 kD 4 kD 2 kD 5. You have just completed a mass spectrometry experiment and you have successfully determined the molecular mass of your 100 amino acid protein. For the experiment, you treated your protein with the enzyme trypsin, which cleaves after all Lys and Arg residues. Your protein has Lys at positions 25, 65 and 82 and Arg at positions 14, and 58. a. How many amino acids would you have in each of the resulting proteolytic fragments? b. Estimate the molecular weight of each of these fragments. (Remember that the mass spec technique would give you exact masses of each of these fragments) c. What information do you gain by cleaving your peptide with trypsin?...
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This note was uploaded on 12/07/2011 for the course BIBC 102 taught by Professor Price during the Spring '02 term at UCSD.
- Spring '02