Major Points, Lecture 14 SS10

Major Points, Lecture 14 SS10 - 11 What does DNA pol I do...

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BSCI105, Freshman Connection Fall 2010 Major Points, Lecture 14 (Chapter 16) 1. What feature(s) of the Watson-Crick model of DNA suggested a mechanism for replication? 2. When does DNA replication occur during the cell cycle? 3. Where does DNA replication start? How does this differ between prokaryotes and eukaryotes? 4. What are three general events that occur during DNA replication? 5. Can you list the seven key proteins listed in Table 16.1? Can you define one major function for each protein during DNA replication? 6. What does Topisomerase do? Why is this a necessary function for DNA replication? 7. What does Helicase do? 8. What prevents single strands of DNA from immediately re-associating and "rewinding?" 9. What is the function of Primase? 10. What does DNA pol III do? What are two limitations on the function of DNA pol III?
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Unformatted text preview: 11. What does DNA pol I do? What does DNA pol I recognize about its target molecule? What are the two sequential steps in the function of DNA pol I? 12. What doe DNA Ligase do? How does it do it? Is this function more important on the leading or lagging strand? 13. Can you label and define all of the key proteins in a figure of a replication fork? 14. What are the two types of DNA synthesis that occur on the leading and lagging strands? 15. What is difference between how DNA is synthesized on the leading and lagging strands? 16. Define the "linear chromosome problem." What is the source of the linear chromosome problem? What is the detrimental outcome of the problem? 17. What is the function of telomeres? How do telomeres fix the linear chromosome problem?...
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