P2_F06ANSPOST - Prelim 2 regrade requests must be submitted...

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Prelim 2 regrade requests must be submitted in writing to Shirley Soule NO LATER THAN 2:00PM on Friday 12/2 (217 Stimson, Mondays & Wednesdays 8:00 AM - 2:00PM, Fridays 10:00AM-2:00 PM). 1. 5' 3' A G A A T T T T A G G G C C C 5' 3' C T A A T T T A A G G G C C C a. (1 point) Name the enzyme that could catalyze the repair of the break in the top strand. (Nelson & Cox, p. 958) DNA Ligase b. (1 point) On the diagram above circle one bond that could be cleaved by initial attack of a 3’ to 5’ exonuclease. (Nelson & Cox, Fig 25-7, p. 955) There are three different 3' ends that could be circled for full credit. (4 points) Name two enzymes from prokaryotes that have this capability. (Nelson & Cox, Table 25-1, p. 956) DNA pol I, II, and III (only two required) (4 points) Name and describe the function of such 3’ to 5’ exonucleases in DNA replication? (Nelson & Cox, p. 955) The function is called proofreading. A nucleotide is checked after it is added. If the nucleotide is not properly hydrogen bonded to the corresponding template nucleotide, it will be excised and replaced. c. (1 point) On the diagram above, place a square around one bond that could be cleaved by initial attack of a 5’ to 3’ exonuclease. (Nelson & Cox, pp. 962, 972) There are also three 5' ends that are susceptible to attack by this exonuclease activity. (4 points) List two roles of the 5’ to 3’ exonuclease activity. (Nelson & Cox, p. 962) RNA primer removal (Nelson & Cox, p. 972) DNA repair (base-excision repair, etc.)
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BioBM330 2nd prelim 10/31/06 Name: ___________________________2 2. a. (2 points) Name and briefly describe the fundamental organizational unit of chromatin. (Nelson & Cox, p. 938-40) Nucleosomes (1 point) are a complex in which the DNA double helix is wrapped around a core of histone proteins (1 point). b. (4 points) Name and/or briefly describe the two higher order organizational structures in chromatin. (Nelson & Cox, p. 942-3) Nucleosomes are packed into a coil that is referred to as the 30 nm fiber. At intervals, this 30 nm fiber is attached to the nuclear scaffold with the scaffold associated regions separated by loops. c. (2 points) What is the function of chromatin remodeling? (Nelson & Cox, p. 1103) The function of chromatin remodeling is to activate otherwise inactive regions of the genome for transcription. (2 points) Name or briefly describe the modification of histone proteins that occurs extensively during chromatin remodeling. (Nelson & Cox, p. 1103) Histone proteins are acetylated during chromatin remodeling 3. a. (2 points) What is meant by processivity? (Nelson & Cox, p. 954) Processivity is a measure of the average number of nucleotides that are added before a polymerase dissociates.
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P2_F06ANSPOST - Prelim 2 regrade requests must be submitted...

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