behavioural_practical_v03

behavioural_practical_v03 - Contextdependent...

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Context-dependent  Context-dependent  effects of diazepam  effects of diazepam  on behaviour in mice on behaviour in mice Lawrence Moon Pat Wallace Please switch off mobiles!
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Goals Goals 1. To critically evaluate design of  in vivo  experiments 2. To become familiar with practical aspects of  in vivo  work 3. To apply appropriate statistical methods to  in vivo  data 4. To experience the effects of neuroactive drugs (!)
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Overview Overview 1. Students will be allocated into groups of five 2. Quick exercise in groups 3. Feedback from groups – students will be asked questions! 4. Introduction to experiments 5. Students will be asked questions to check understanding! 6. Demo of behaviours to score 7. Students run first experiment -> two groups of 15 8. Demo of how to scruff and inject mice. 9. Students run second experiment 10.Lunch 11. Statistical analysis 12. Discussion of results
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Allocate students into groups Allocate students into groups
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A quick exercise to test prior knowledge. A quick exercise to test prior knowledge. Discuss in your group of three: Discuss in your group of three: 1.What family of drugs does  diazepam (Valium) belong to? 2.What can diazepam be used to  treat / what are the effects of  diazepam in humans?   (I’ll be asking members of each group  (I’ll be asking members of each group  to provide replies. But don’t worry if  to provide replies. But don’t worry if  you don’t know the answers!) you don’t know the answers!)
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A quick exercise to test prior knowledge. A quick exercise to test prior knowledge. Discuss in your group: Discuss in your group: 1.What family of drugs does diazepam (Valium)  belong to? BENZODIAZEPINES 2.What can diazepam be used to treat? ANXIETY ( e.g.  as a sedative / anxiolytic) INSOMNIA MUSCLE SPASM ALCOHOL WITHDRAWAL SEIZURES
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Introduction to experiments Introduction to experiments Aim is to show that the same dose of diazepam in  mice can produce behavioural effects that are  either sedative or anxiolytic depending on the  testing environment: • Boxmaze exploration • Locomotor activity measurement
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First experiment: Boxmaze exploration First experiment: Boxmaze exploration LOW WALLS MEDIUM WALLS UNCROSSABLE WALLS  PERSPEX LID GOES  ON TOP Imagine you are this mouse.             What might you do? 
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Hypothesis to test: diazepam affects  behaviours in the boxmaze Experimental design:  I’ll ask for class feedback on this! Experimental design:  I’ll ask for class feedback on this!
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This note was uploaded on 12/09/2011 for the course STATS 221 taught by Professor Nielson during the Fall '10 term at BYU.

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behavioural_practical_v03 - Contextdependent...

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