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Unformatted text preview: - Exercises 3-10, 3-13, 3-7, and Supplement pg. 4 1. For best results, should a fresh culture be used when performing an endospore stain? Why or why not? 2. What is the purpose of covering the smear with bibulous paper when performing the endospore stain? 3. What are two possible problems that are encountered when performing a flagella stain? 4. How would a Gram positive cell appear immediately after decolorization? A gram negative cell? Why? 5. Why is the Gram stain more helpful than a simple stain when identifying an unknown organism? 6. Give two reasons why a Gram stain may not yield clear results. 7. What is the purpose of the mordant when Gram staining? Why does this not affect Gram-negative cells. 8. What is the purpose of the low agar percentage in the motility plates? How does this affect incubation? 9. What do you expect the motility results to be for the motile and non-motile strains of E. coli ?...
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- Fall '08