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Unformatted text preview: organisms that arent esculin-positive in the presence of bile. 4. Esculin was hydrolyzed to beta-D glucose and Esculetin, forming a dark brown iron precipitate that is deposited in the media. 5. The classical method took 48 hours and tested for the presence of benzoic acid in hippurate hydrolysis. The rapid method is two and a half hours, uses the enzyme hippuricase and tests for the presence of glycine. 6. MacConkey allows for differentiation among members of the Enerobacteriaceae family because lactose fermenters turn pink while others stay colorless. 7. All three innoculations require more time to grow for results to be seen, so the results can be recorded on day 2. They also differentiate and select among large groups of bacterial families and this helps preliminarily to narrow down the what the unknowns could be....
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This note was uploaded on 12/07/2011 for the course BIOL 351 taught by Professor Sig during the Fall '08 term at Texas A&M.
- Fall '08