L-6 - 3) Tertiary structure (3°) Local domains (e.g.,...

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Unformatted text preview: 3) Tertiary structure (3°) Local domains (e.g., eM-helix, sheets, etc.) folding in on each other. A protein structure is made up of one or more domains (compact, locally folded regions). A tertiary structure is not a static and rigid structure. The amino acid sequence (the primary structure) determines the tertiary structure. C. Anfinsen’s experiment: D6ma‘turafion : .033t'.|’€ km+eia ) ? has)!“ Cor.) Cte. 've r'l / - ) act» 1 0mm cue ('3‘wa mjnuchm) Rema‘Eura‘Bm : Few-won 19A? JflhatH/‘ffla thfm.‘ Pa-¥a~m 9 nai‘ivc cm) [Moi-9i... )yence: Actually, rlbonuclease refolds In only one minute. Nevertheless, the folding process proceeds through distinct steps. 1) nucleation «a, 2) structural consolidation 3) final rearrangements What would have happened If lnsulln had been used lnste‘ad of rlbonulease? ® 1) Globular proteins have distinct insides and outsides 2) P-Sheets are usually twisted or wrapped into a barrel structure. 3) Turns. 4) "Random coil" regions: actually, irregular structUred regions. 5) Prosthetic groups are often present. 6) FOLDING IS THERMODYNAMICALLY FAVORED. Force; af'fec'l'ing fie {crew/'7 (I'm. .1.) Elec'i’ros‘f'a‘l'h in+erac+io>ws (6.3-) 5‘3)£— A/W4395) o . ...._ cHz-c\/Ze HsNe—"CH-L—H. \~o mot? e1cizec2": 01A ox {'12} +2702 07L , {A ‘l‘e/‘ac ~(""0 ‘n aH—era‘f‘m‘ o7£ o)ie)ec‘Z‘/‘fc CW5+M71 Cax also --. In +23 .sec‘hdx ) we CM a/So {ACAJP a) Acfu In}: a e Azeth A) leo )9 - all-f0 )e {a zLe/acz‘v’ou c) J70}? — {no/urea, Jil‘aa/e )hfi/arl‘w 02) ea. 2) Hydrogen banal/)9 @ 0H3)» bffWGEh $3.46 Jain: 34-] Auk“ e’e') . /. ...—CH‘-O'H Mil)“ 0=C \. 3’30 Infor‘l'ax‘t': @ ‘3) Vin Aer V1133): "(cortex 9 Weak {a ‘l-erac‘l-rn‘ mevfflfle)“$) aha/A7 a)oék)2/‘ cafe/3y are Jaye/y Pack?! N o. 3 Led/M18 or ~12k2’ 4-) 4r Angling , ar0%3£:‘c amino acid! I»??? Clair: SJL’ a c L-fnj 6.) Covalchf Abiding elemen‘éx - #or examP/e) a’f$u)7£l‘de Arfdzje‘c "Afo‘api'Ed’ 1'73 Alec/2r A‘l‘Pr aci’fmx or H7JI‘OPAOLJ.‘ [31 {Trad-{43“ " sequesi‘e/finfl o‘ff' max/0°)” 9'19 clap! 2wa7 7&0» fie fo/ar enuf/‘Onaen .. [prof-Pin: 2‘an a 513+}.n4'lt fall-4’9 M witch/we. "" a wajOr J9 *PFKJ'nfng fn‘fi/Hence ox #_ ‘ 1Ler+§3r~7 si’ruc‘zLu/‘e Consider f/xe calm/{JFIW lei-ween fie #OUGJ aoJ M1A°)A/€0) 700/3» 071‘ a frofefn: # A a uNFowsa F0 Logo COWPDSEJ 070 a air 2)_TA§ A H° ;S flagging [ecausc at . -. gm— wHA‘T #80147 As”? 2m» chi-Arnie Waite, “Lam”: ‘hfi’n'bu‘as‘eo’ in file 7473,.)- ‘A’w Maine/‘91. Hyah‘oflozft 812(9‘9' Jr‘PV/z‘ej 17" GE 4) Quaternary structure (4") ’ Not all proteins have a quaternary structure. Multisubunit proteins: i.e., more than one polypeptide chain. forces influencing quaternary structure: 1) heterologous Interactions: isologous interactions: D; Lea,- I h 3 5y ) D,7 I’m/{Ag a» 7I-7ZDXJ 330.; W326} '77 {'WO’IZOM’ axe; examp/e : a. i—ef/‘a her I) 2 z PURIFICATION OF PROTEINS All relying on specific proper’ries dialysis and ul’rrafil’rrafion .cen’rrifugo’rion fracfional precipi’ra’rion chroma’rography ion‘éxchange HPLC gel fiIIra’rion affinin elec’rrophoresis esp” SDS—PAGE isoelec’rric focusing ...
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This note was uploaded on 12/09/2011 for the course BCH 4024 taught by Professor Allison during the Spring '08 term at University of Florida.

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L-6 - 3) Tertiary structure (3°) Local domains (e.g.,...

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