Old_Exam_3__2008 Summer_

Old_Exam_3__2008 Summer_ - BCH 4024 INTRODUCTION TO...

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Unformatted text preview: BCH 4024 INTRODUCTION TO BIOCHEMISTRY AND MOLECULAR BIOLOGY R. Donald Allison, Ph.D. THIRD EXAMINATION: JULY 17, 2008 Please PRINT your last name neatly on the answer sheet, followed by your initials. Fill-in your UF ID Number legibly and accurately. You must be careful in doing this. If you are unsure of how to properly encode your number and name onto the answer sheet, ask a proctor for assistance. If the answer sheet is filled~in incorrectly, your sheet will be lost in cyber-space! Mark the most-nearly—correct answer on the sheet by completely filling-in the appropriate box with the correct type of pencil. If you do not have the correct type of pencil, one can be provided for you. Each question is worth two points. You have two hours to work on the examination. At the end of that time, all answer sheets will be collected. If you need assistance, RAISE YOUR HAND AND WAIT IN YOUR SEAT FOR A PROCTOR. AT NO TIME SHOULD YOU RAISE YOUR EXAMINATION OR YOUR ANSWER SHEET OFF YOUR DESK! Before the start of the examination, remove all notes, books, etc., from your desk. Please keep at least one vacant seat between you and any classmate. DO NOT OPEN THE EXAMINATION UNTIL YOU ARE TOLD TO DO SO BY THE PROCTOR. Take a breath, relax, and do your best. Which of the following corresponds to oleic acid? A) 16:0 B) 16:1 C) 18:0 D) 18:1 E) 20:4 Which of the following contains a trans double bond? A) linoleic acid B) elaidic acid C) arachidonic acid D) oleic acid E) stearic acid Which of the following contains two moles of phosphate per mole of lipid? A) phosphatidylcholine B) sphingomyelin C) cardiolipin D) prostaglandin E; E) phosphatidic acid Which of the following is an example of a peripheral membrane protein? A) fatty-acyl—COA synthetase B) carnitine acyltransferase II C) enoyl—COA hydratase D) camitine translocase E) cytochrome c The cmc value for a lipid corresponds to : A) the temperature at which the lipid aggregates, B) the critical melting temperature for a membrane containing that lipid. C) the concentration at which a micelle will form. D) the upper limit of the parachor for a lipid (that is, the surface tension divided by the molecular weight). E) none of the above Which of the following is true of bile salts? A) They function as surfactants or detergents in lipid degradation. B) They are precursors in cholesterol bicsynthesis. C) They activate acetyl-CoA carboxylase. D) They inhibit hormone-sensitive lipase. E) They inhibit insulin binding. 7. 10. ll. 12. Which of the following will stimulate the activity of hormone—sensitive lipase? A) B) C) D) E) insulin. glucagon estrogen. prostaglandin A2. glycerol. Which of the following is an intermediate in the formation of fatty-acyl—CoA from a fatty-acid, coenzyme A, and ATP? (In a single, enzyme—catalyzed reaction?) A) B) C) D) E) fatty—enoyl-CoA fatty-acyluadenyl ate hydroxy—enoyl—COA fatty—acyl—camitine none of the above Which of the following enzymes is located in the outer mitochondrial membrane? A) B) C) D) E) carnitine acyltransferase I (CAT I) camitine translocase succinyl—CoA synthetase enoyl-CoA hydratase of Bwoxidation fatty acid synthase Certain foods contain very long—chain, saturated fatty acids. What is the maximum net number of ATP molecules that can be made from a 26-carbon, straight—chain, saturated fatty acids? A) 197 B) 214 C) 216 D) 219 E) 221 Which of the following steps in B~oxidation utilizes NAD+ as a substrate? A) B) C) D) E) the enzyme that produces acetyl-CoA the enzyme that produces the fatty-enoyl—COA derivative the enzyme that produces the 3-ketoacyl—C0A derivative the enzyme that produces the 3—hydroxy—acyl~Coa derivative none of the above Phytamic acid, a branched-chain, fatty acid, is degraded utilizing A) B) C) D) E) hydro gen-peroxide dependent pathway. (ta-oxidation. a pathway which oxidizes every carbon except the beta-carbon. (ii-oxidation biotin 13. 14. 15. 16. 17. 18. Which of the following is an intermediate in the conversion of propionyl-CoA to succinyl—CoA? A) mevalonyl-CoA B) acetoacetyl-COA C) hydroxyinethylglutaryl-COA D) malonyleoA E) methylmalonyl—COA Which ofthe following is a ketone body circulating in blood plasma? A) hydroxyglutarate B) acetoacetate C) malonate D) propionate E) acetate The enzyme that produces the first ketone body in ketogenesis uses as a substrate. A) 3-hydroxybutyrate B) acetyl-CoA C) 3—hydroxy—3 -methylglutary1—COA D) acetone E) propionyl—COA The rate-determining step in fatty-acid biosynthesis is activated allosterically by: A) malonate. B) fatty acids. C) pantothenate. D) citrate. E) NADPH The step that utiliZes the first NADPH in the fatty-acid synthase complex produces: (Below, "ACP" refers to the acyl-carrier protein) A) acyl-ACP B) 3-hydroxyacyI—ACP C) 3—ketoacyl—ACP D) 2—enoy1—ACP E) palmitoyl—CoA Which of the following metabolites directly provides the acetyl-CoA needed for fatty-acid biosynthesis (in one enzyme~cata1yzed reaction)? A) citrate. B) malonate C) ac eto acetate D) hydroxyglutarate E) pyruvate 19. 20. 21. 22. 23. 24. Which of the following nucleotides is utilized in the bicsynthesis ofphosphatidylcholine from choline? A) ATP B) GTP C) UTP D) CTP E) ADP The production of is the rate-determining step in cholesterol biosynthesis: A) mevalonate B) squalene C) lanosterol D) famesyl-pyrophosphate E) isopentenyl—pwophosphate What is the net number ofNADPI—I used to synthesize squalene? A) 2 B) 12 C) 13 D) 18 E) 19 Which of the following particles carries cholesterol from the intestine to other tissues? A) micellular fatty acids B) chylomicrons C) LDL D) HDL E) none ofthe above Which of the following vitamins is used in reaction resulting in carboxylations? A) thiamin B) biotin C) folic acid D) panthothenic acid E) cobalamin (Vitamin B12) Beriberi is caused due to a nutritional deficiency of : A) thiamin B) riboflavin C) lipoic acid D) pyridoxine E) niacin 25. Which of the coenzymes below is synthesized from panthothenic acid? A) FAD B) NAD C) coenzyme A D) tetrahydrobiopterin E) inositol 26. Coenzymes derived from vitamin B12 participate in methyl/transfer reactions and reactions. A) decarboxylation B) methylene transfer C) oxidation/reduction D) ctr/trans isomerization E) rearrangement 27. Ingestions of will result in a biotin deficiency. A) malonate B) inositol C) avidin D) any ketone body E) choline 28. Which of the following compounds is found covalently bound to a protein? A) NAD 13) FAD C) lipoic acid D) coenzyme A E) thiamin pyrophosphate 29. Which of the following is a nutritionally essential amino acid in mammals? A) proline. B) leucine. C) glutamine. D) serine E) cysteine 30. Which of the following releases histidine upon the hydrolysis of a peptide bond? A) vanillin B) mevalonate C) creatine D) carnosine E) captopri} 31. 32. 33. 34. 35. The daily requirement for in the diet. is lessened by the presence of a A) phenylalanine; tryptophan B) leucine; isoleucine C) threonine; serine D) asparagine; glutamine E) methionine; cysteine Which one of the following amino acids does not have an aminotransferase (transaminase) in mammals? A) histidine B) tyrosine C) threonine. D) leucine E) methionine A certain aminotransferase has an internal aldimine (i.e., internal Schiff base) linking the coenzyme to : A) any amino group B) lysine C) ketinine D) leucine E) glutamine Cirrhosis, a disease of the liver, is often accompanied by elevated plasma concentrations of: A) glutamine B) hemoglobin C) creatine D) aspartate aminotransferasis E) phosphatidylcholine Mammalian glutamate dehydrogenase is inhibited by elevated concentrations of : A) GDP B) NAD+ C) ATP D) citrate E) isocitrate 36. 37. 38. 39. 40. Which ofthe following is the immediate precursor in the biosynthesis of aspartate? A) ox aloacetate B) methylm alonate C) u—ketoglutarate D) 3 -phosphoglycerate E) citrate The primary means for the biosynthesis of glycine requires the presence of a co—enzyme derived from : A) niacin B) riboflavin C) folic acid D) thiamin E) tocopheroll Which of the following is required for the conversion of phenylalanine to tyrosine? A) coenzyme A B) thiamin pyrophosphate C) tetrahydrofolate D) tetrahydrobiopterin E) hydroxyproline The primary nitrogen donor in mammals is: A) ammonia B) glutamine C) asParagine D) arginine E) guanine Which of the following supplies the carbon atoms in the biosynthesis of cysteine? A) methionine B) threonine. C) d—ketobutyrate D) serine E) d-aminobutyrate 41. Which of the following produces a metabolite that is a major methyl donor? A) valine B) alanine C) serine D) methionine E) leucine 42. Elevated levels of ammonia in the blood plasma is usually accompanied by elevated levels of: A) glycine B) glutamate C) methionine D) urea E) alanine 43. The first step in the urea cycle, catalyzed by carbamoyl phosphate synthetase I, is activated by elevated levels of: A) urea B) UTP C) N—acetyl glutamate D) acetyl-CoA E) omithine 44. The atom labelled below with an asten'sk (*) originates from: N'H + l ~00C _ (“2.4m -NH- C 'NH ’(C”2)3'CH NH: I f coo“ f COO- A) ammonia B) creatine C) urea D) omithine E) aspartate 45. 46. 47. 48. 49. 50. Which of the enzyme-catalyzed reactions below also produces pyrophosphate (Ho3 P—OfiPO3H')? A) B) C) D) E) There is an enzyme, not in the urea cycle, that converts arginine to citrulline and A) B) C) D) E) Which of the following is both a glycogenic and a ketogenic amino acid? A) B) C) D) E) the formation of argininosuccinate the formation of urea the formation of carbarnoy] phosphate the formation of citrulline the formation of ornithine urea ammonia hydrogen peroxide nitric oxide nitrate tryptophan methionine histidine valine leucine Which of the following amino acids degrades to d—ketoglutarate in a pathway described in class? A) alanine B) phenylalanine C) asparagine D) histidine E) valine Alcaptonuria is associated with a deficiency of an enzyme in the pathway. A) isoleucine B) tryptophan C) tyrosine D) histidine E) asparagine The "end-product" in the degradation of valine is : A) B) C) D) E) ac etyl—CoA succinyl—CoA fumaryl—CoA aceto acetyl—CoA hypo glycyl-CoA degradation ...
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This note was uploaded on 12/09/2011 for the course BCH 4024 taught by Professor Allison during the Spring '08 term at University of Florida.

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Old_Exam_3__2008 Summer_ - BCH 4024 INTRODUCTION TO...

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