JINALBEN PATEL0632747Lab 3 and 4 Assignment This value of this assignment is 36 marks and it is worth 10% of your final grade. Please type your answers in the spaces provided. Table 1. Volumes that will be used to generate solutions containing a known concentration of BSA (7 marks)12345678LabelBase concVolume ofstock BSAVolume of1.15M NaClTotal vol-umeA595nm tube set 1A595nm tube set 2A595nm averageTube 100100100000Tube 2 255951000.200.150.175Tube 3 5010901000.170.220.195Tube 47515851000.200.260.230Tube 510020801000.300.330.315Calculations (2 marks). Show sample calculations for columns 3 and 4.
JINALBEN PATEL0632747100*100/50020ulColumn 4 we have to make a total of 100 ul of volume, so if we take 5 ( of volume stock of BSA then we need (100-5) which is 95 ul of volume of 0.15M NaCl.Volume stock of BSA of 10 ul will be (100-10) which is 90 ulTABEL 2: using standard curve to determine the protein concentration of an unknown sample (6marks)12345LabelA 595 nmProtein conch in diluted sampleDilution factor