BIOL2070_JINALBEN_PATEL_LAB3_0632747.docx - JINALBEN PATEL...

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JINALBEN PATEL 0632747 Lab 3 and 4 Assignment This value of this assignment is 36 marks and it is worth 10% of your final grade. Please type your answers in the spaces provided. Table 1. Volumes that will be used to generate solutions containing a known concentration of BSA (7 marks)12345678LabelBase concVolume ofstock BSAVolume of1.15M NaClTotal vol-umeA595nm tube set 1A595nm tube set 2A595nm averageTube 100100100000Tube 2 255951000.200.150.175Tube 3 5010901000.170.220.195Tube 47515851000.200.260.230Tube 510020801000.300.330.315Calculations (2 marks). Show sample calculations for columns 3 and 4.
JINALBEN PATEL 0632747 100*100/500 20ul Column 4 we have to make a total of 100 ul of volume, so if we take 5 ( of volume stock of BSA then we need (100-5) which is 95 ul of volume of 0.15M NaCl. Volume stock of BSA of 10 ul will be (100-10) which is 90 ul TABEL 2: using standard curve to determine the protein concentration of an unknown sample (6marks) 1 2 3 4 5 Label A 595 nm Protein conch in diluted sample Dilution factor

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