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Unformatted text preview: me as I was able to see it hands-on. I learned a lot in this lab about G-C content and what it means for the strength of the DNA it is in. This was mostly done through observation of Tm and a thermal denaturation curve. We have learned in lecture that the 3 hydrogen bonds in G-C strengthen the DNA compared to the 2 hydrogen bonds between A and T. This, in turn, means that DNA with higher G-C content will require more heat to fully denature and thus have a higher Tm. I saw this after calculating the Xgc for each species and then plugging it into the Tm equation. Indeed, the one with the highest G-C content— Micrococcus lysodeikticus with 71.48%—had the highest Tm of 89.99 degrees. The one with the lowest—salmon testes with 46.70%--had the lowest Tm of 79.05 degrees. This lab just goes to show how big of an impact non-covalent, weak hydrogen bonds can have on a molecule....
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- Fall '08
- DNA, Calf Thymus, G-C content, Salmon Testes, thermal denaturation curve