5 - ANNOUNCEMENTS: LECTURE 5 1. QUIZ WED 9/8. MORE INFO AT...

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ANNOUNCEMENTS: FRIDAY'S LECTURE WHAT TO LEARN FROM AA SEQ WITHOUT 3D STRUCTURE : UNKNOWN PROTEIN-- LOOK FOR AA SEQ MATCH WITH KNOWN PROTEINS FIND EVOLUTIONARY RELATIONSHIPS TRY TO PREDICT 3D STRUCTURE(!) 3D STRUCTURE: INTRO TO X-RAY DIFFRACTION TODAY'S LECTURE WHAT ARE THE "RULES" FOR PROTEIN STRUCTURE? 4. REVIEW SESSION (MAINLY HOMEWORK PROBLEMS) IN ROCKEFELLER 104 TUE 9/7 FROM 3 - 4:10 PM 3. OFFICE HOURS: TUESDAY 9/7 IN BIOTECH 202: 8:30AM - 2:45PM LECTURE 5 5. INTERESTED IN NUTRITION, HEALTH, COMMUNITY SERVICE? MARCH OF DIMES AT CU 1. QUIZ WED 9/8. MORE INFO AT THE END OF TODAY'S LECTURE. BASICS OF X-RAY DIFFRACTION 6 CATEGORIES OF FORCES; CONSTRAINTS AROUND THE PEPTIDE BOND 6. ANY INTERESTED STUDENTS: “DID YOU FIND A LAB RESEARCH POSITION? WHAT IS EXPECTED OF A NEWBIE RESEARCHER?!", 2:55 - 4:10PM, TODAY IN COMSTOCK B108.
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THE DIFFRACTION EXPERIMENT MEASURE DIFFRACTED X-RAY WAVES : DIRECTION INTENSITY X-RAY WAVELENGTH PHASE PROTEIN CRYSTAL X-RAY BEAM X-RAY WAVES DIFFRACTED BY PROTEIN CRYSTAL DETECTOR, e.g. CCD CAMERA OH NO! NO MATTER WHAT THE PHASE , THE SAME SPOT INTENSITY APPEARS ON THE DETECTOR: LOSE ALL PHASE INFO! CONTAIN ALL INFO NEEDED TO SOLVE 3D ARRANGEMENT OF ATOMS! TOOK 30 YEARS TO SOLVE "PHASE PROBLEM" CURRENT LIMITING FACTOR FOR SOLVING PROTEIN 3D BY X-RAY IS GOOD CRYSTALS
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MANY DIFFERENT WAYS TO SHOW PROTEIN STRUCTURE F HELIX E HELIX HEME PROXIMAL HIS DISTAL HIS FOURIER TRANSFORM SPOT DATA INTO e - DENSITY STICKS SPHERES CARTOON RIBBON
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5 - ANNOUNCEMENTS: LECTURE 5 1. QUIZ WED 9/8. MORE INFO AT...

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