Optimal Reaction Rate of Alkaline Phosphatase Enzyme

Optimal Reaction Rate of Alkaline Phosphatase Enzyme - The...

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The Effects of Temperature and pH on the Optimal Reaction Rate of Alkaline Phosphatase Enzyme Abstract The productivity of an enzyme was measured by determining the optimal pH and temperature in order to form the maximum amount of product. We hypothesized that since alkaline phosphatase enzymes, as seen by their name, work best in alkaline solutions and in high temperatures , the optimal pH would be basic for an enzyme to function and that the optimal temperature would be close to normal body temperature. Six test tubes were filled with water and substrate as well as a different pH of buffer. After, enzyme was added and recorded for the amount of product formed. Likewise, eight test tubes were filled with buffer and substrate and placed in different temperatures. After, enzyme was added and recorded for the amount of product formed. It was determined that the optimal pH for an enzyme to react and produce the most product was at a pH of 10. The reaction rate decreased as the pH of buffer decreased from 10, and at a higher pH, the reaction rate also decreased. The optimal temperature for an enzyme to react and produce the most product was at a temperature of 37 degrees Celsius, and that while an enzyme still forms product above and below these standards, the enzyme will not react to full potential unless at their optimum level. Introduction Enzymes are proteins that speed up the chemical reaction. They lower the activation energy needed to start a reaction. A substrate is a molecule upon which an enzyme acts. Enzymes catalyze chemical reactions involving the substrates. In the case of a single substrate, the substrate binds with the enzyme active site , and an enzyme-substrate complex is formed. The
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substrate is transformed into one or more products , which are then released from the active site. The pH of a chemical reaction can affect enzymes depending on its value. A narrow pH range enzyme activity can be reversible. High pH values, whether they are acidic or basic, can cause the enzyme to be completely inactive through a process called denaturation . This behavior involves the change in structure of the enzyme, which no longer allows the substrate to bind to the active site of the enzyme, rendering the enzyme useless. The pH level can also change the shape of the active site in an enzyme. Te mperature affects enzyme activity by changing the three-dimensional structure of the enzyme. A higher temperature generally results in an increase in enzyme activity. Cold temperature, on the other hand, slows down enzyme activity by decreasing molecular motion. (Kaiser, 2001) The purpose of the experiment is to conduct a study using a Spec 20 to measure absorbance of the substrate and product on enzyme
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This note was uploaded on 12/11/2011 for the course BIO 101 taught by Professor Martin during the Spring '08 term at Rutgers.

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Optimal Reaction Rate of Alkaline Phosphatase Enzyme - The...

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